Anti-Fluorescein antibody (Rhodamine) (ab7253)

Overview

  • Product name
    Anti-Fluorescein antibody (Rhodamine)
    See all Fluorescein primary antibodies
  • Description
    Goat polyclonal to Fluorescein (Rhodamine)
  • Conjugation
    Rhodamine. Ex: 550nm, Em: 570nm
  • Tested applications
    Suitable for: Immunomicroscopy, Flow Cytmore details
  • Immunogen

    Fluorescein conjugated to Goat IgG

  • General notes

    Tetramethylrhodamine isothiocyanante (TRITC) (Molecular Weight 444 daltons) Absorption Wavelength: 550 nm Emission Wavelength: 570 nm Fluorochrome/Protein Ratio: 1.6 moles TRITC per mole of Goat IgG.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C.
  • Storage buffer
    pH: 7.60
    Preservative: 0.01% Sodium azide
    Constituents: 0.88% Sodium chloride, 1% BSA, 0.42% Potassium phosphate
  • Concentration information loading...
  • Purity
    IgG fraction
  • Purification notes
    This product was prepared from monospecific antiserum by immunoaffinity chromatography using Fluorescein IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab7253 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Immunomicroscopy
Flow Cyt
  • Application notes
    Immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
  • Target

    • Relevance
      Fluorescein is a fluorophore commonly used to label proteins - protein-fluorescein conjugates are not usually susceptible to precipitation. In addition to its relatively high absorptivity, excellent fluorescence quantum yield and good water solubility, fluorescein has an excitation maximum of 494 nm that closely matches the 488 nm spectral line of the argon-ion laser, making it an important fluorophore for confocal laser-scanning microscopy and flow cytometry applications. Its fluorescence is pH sensitive and is significantly reduced below pH 7. Fluorescein emits most strongly between 500 and 550 nm, but it has a relatively broad emission spectrum reaching to over 600 nm. Several derivatives of fluorescein are commonly used, including FITC (fluorescein isothiocyanate), carboxylates and succinimidyl esters.
    • Alternative names
      • FI antibody
      • FITC antibody
      • fluorescein isothyocianate antibody
      • fluorescyl antibody

    References for Anti-Fluorescein antibody (Rhodamine) (ab7253)

    ab7253 has not yet been referenced specifically in any publications.

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    Thank you for your inquiry.

    The antibody has not been tested for binding to free flourescein, but some binding to the free form is expected since the antibody is polyclonal. The antibody is tested against flourescein conjugated protein. I hea...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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