Rhodamine Anti-Fluorescein antibody (ab7253)
Key features and details
- Rhodamine Goat polyclonal to Fluorescein
- Suitable for: Immunomicroscopy, Flow Cyt
- Reacts with: Species independent
- Conjugation: Rhodamine. Ex: 550nm, Em: 570nm
- Isotype: IgG
Overview
-
Product name
Rhodamine Anti-Fluorescein antibody
See all Fluorescein primary antibodies -
Description
Rhodamine Goat polyclonal to Fluorescein -
Host species
Goat -
Conjugation
Rhodamine. Ex: 550nm, Em: 570nm -
Tested applications
Suitable for: Immunomicroscopy, Flow Cytmore details -
Species reactivity
Reacts with: Species independent -
Immunogen
Chemical/ Small Molecule corresponding to Fluorescein. Fluorescein conjugated to Goat IgG
-
General notes
Tetramethylrhodamine isothiocyanante (TRITC) (Molecular Weight 444 daltons) Absorption Wavelength: 550 nm Emission Wavelength: 570 nm Fluorochrome/Protein Ratio: 1.6 moles TRITC per mole of Goat IgG.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 6.50
Preservative: 0.01% Sodium azide
Constituents: 0.88% Sodium chloride, 1% BSA, 0.42% Tripotassium orthophosphate -
Concentration information loading...
-
Purity
Affinity purified -
Purification notes
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Fluorescein IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab7253 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
Immunomicroscopy | ||
Flow Cyt |
Notes |
---|
Target
-
Relevance
Fluorescein is a fluorophore commonly used to label proteins - protein-fluorescein conjugates are not usually susceptible to precipitation. In addition to its relatively high absorptivity, excellent fluorescence quantum yield and good water solubility, fluorescein has an excitation maximum of 494 nm that closely matches the 488 nm spectral line of the argon-ion laser, making it an important fluorophore for confocal laser-scanning microscopy and flow cytometry applications. Its fluorescence is pH sensitive and is significantly reduced below pH 7. Fluorescein emits most strongly between 500 and 550 nm, but it has a relatively broad emission spectrum reaching to over 600 nm. Several derivatives of fluorescein are commonly used, including FITC (fluorescein isothiocyanate), carboxylates and succinimidyl esters. -
Alternative names
- FI antibody
- FITC antibody
- fluorescein isothyocianate antibody
- fluorescyl antibody
Datasheets and documents
-
SDS download
-
Datasheet download
References (0)
ab7253 has not yet been referenced specifically in any publications.