Anti-Fluorescein antibody (Texas Red) (ab6654)

Overview

• Product name
  Anti-Fluorescein antibody (Texas Red)
  See all Fluorescein primary antibodies
• Description
  Goat polyclonal to Fluorescein (Texas Red)
• Conjugation
  Texas Red ®. Ex: 596nm, Em: 620nm
• Tested applications
  Suitable for: ELISA, Flow Cyt, WB, ICC/IFmore details
• Immunogen

  Fluorescein conjugated to Goat IgG

• General notes

  Texas Red^TM is a registered trademark of Molecular Probes Inc.

  Texas Red^TM Sulfonyl Chloride (Molecular Weight 625 daltons) Absorption Wavelength: 596 nm Emission Wavelength: 620 nm Fluorochrome/Protein Ratio: 2.9 moles Texas Red^TM per mole of Goat IgG.

Properties

• Form
  Liquid
• Storage instructions
  Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
• Storage buffer
  pH: 7.20
  Preservative: 0.01% Sodium azide
  Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride, 1% BSA
• Concentration information loading...
• Purity
  IgG fraction
• Purification notes
  This product was prepared from monospecific antiserum by immunoaffinity chromatography using Fluorescein IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
• Clonality
  Polyclonal
• Isotype
  IgG
• Research areas
  • Tags & Cell Markers
  • Epitope Tags
  • Conjugates

Associated products

• Compatible Secondaries
  • Donkey Anti-Goat IgG H&L (Alexa Fluor® 488) (ab150129)
  • Donkey Anti-Goat IgG H&L (HRP) (ab205723)

Applications

Target

• Relevance
  Fluorescein is a fluorophore commonly used to label proteins - protein-fluorescein conjugates are not usually susceptible to precipitation. In addition to its relatively high absorptivity, excellent fluorescence quantum yield and good water solubility, fluorescein has an excitation maximum of 494 nm that closely matches the 488 nm spectral line of the argon-ion laser, making it an important fluorophore for confocal laser-scanning microscopy and flow cytometry applications. Its fluorescence is pH sensitive and is significantly reduced below pH 7. Fluorescein emits most strongly between 500 and 550 nm, but it has a relatively broad emission spectrum reaching to over 600 nm. Several derivatives of fluorescein are commonly used, including FITC (fluorescein isothiocyanate), carboxylates and succinimidyl esters.
• Alternative names
  • FI antibody
  • FITC antibody
  • fluorescein isothyocianate antibody
  • fluorescyl antibody

Protocols

• Flow cytometry protocols
• Immunocytochemistry & immunofluorescence protocols
• Western blot protocols