Overview

  • Product name
    Fluoxetine hydrochloride
  • Description
    5-HT reuptake inhibitor
  • Biological description
    Potent, selective 5-HT reuptake inhibitor. Antidepressant in vivo. Binds to rat and human 5-HT transporters with Ki values of 2 and 0.9-20 nM, respectively. Shows around 150-900-fold selectivity over 5-HT1A, 5-HT2A, H1, α1, α2-adrenergic and muscarinic receptors.
  • Purity
    > 99%

Properties

Applications

Our Abpromise guarantee covers the use of ab120077 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Functional Studies Use at an assay dependent concentration.

Images

  • ab66047 staining serotonin in SKNSH cells treated with fluoxetine hydrochloride (ab120077), by ICC/IF. Increase of serotonin expression correlates with increased concentration of fluoxetine hydrochloride, as described in literature.
    The cells were incubated at 37°C for 24h in media containing different concentrations of ab120077 (fluoxetine hydrochloride) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab66047 (5 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 donkey anti-goat polyclonal antibody (ab96931) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.

References

This product has been referenced in:
  • Owens MJ  et al. Neurotransmitter receptor and transporter binding profile of antidepressants and their metabolites. J Pharmacol Exp Ther 283:1305-22 (1997). Read more (PubMed: 9400006) »
  • Fuller RW  et al. Fluoxetine, a selective inhibitor of serotonin uptake. Med Res Rev 11:17-34 (1991). Read more (PubMed: 1994152) »
  • Wong DT  et al. Fluoxetine and its two enantiomers as selective serotonin uptake inhibitors. Acta Pharm Nord 2:171-80 (1990). Read more (PubMed: 2200429) »

See all 4 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE, NOT FOR USE IN HUMANS"

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