Overview

  • Product name
    Anti-FLYWCH2 antibody
  • Description
    Rabbit polyclonal to FLYWCH2
  • Tested applications
    Suitable for: IHC-P, WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    antigen sequence: SKDSTKVAGA KRKGVHCVMS LGVPGPATLA KALLQTHPEA QRAIEAAPQE PEQKRSRQDP GTDRTEDSGL AAGPPEAAGE NFA, corresponding to amino acids 48-130 of Human FLYWCH2.

  • Positive control
    • Human prostate tissue; FLYWCH2 over-expression HEK293T lysate.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab122712 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/250 - 1/500.
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

Target

Images

  • Immunofluorescent staining of Human cell line A-431 shows positivity in nucleus but not nucleoli. Recommended concentration of ab122712 1-4 µg/ml. Cells treated with PFA/Triton X-100.
  • ab122712, at 1/50 dilution, staining FLYWCH2 in paraffin-embedded Human prostate tissue by Immunohistochemistry.
  • All lanes : Anti-FLYWCH2 antibody (ab122712) at 1/250 dilution

    Lane 1 : Negative control (vector only transfected HEK293T lysate)
    Lane 2 : FLYWCH2 over-expression HEK293T lysate, Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa)

References

ab122712 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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