Overview

  • Product name
    Anti-FMN1 antibody
  • Description
    Mouse polyclonal to FMN1
  • Specificity
    This antibody is reactive against mammalian transfected lysate.
  • Tested applications
    Suitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    FMN1 (AAI03693.1, 1 a.a. ~ 503 a.a) full-length human protein.

  • Positive control
    • FMN1 transfected 293T cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab68058 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 85 kDa.
ICC/IF Use at an assay dependent concentration.

Target

  • Relevance
    FMN1 plays a role in the formation of adherens junction and the polymerization of linear actin cables. Interacts with alpha-catenin and may interact with tubulin
  • Cellular localization
    Nucleus. Cytoplasm. Cell junction, adherens junction; Peripheral membrane protein; Cytoplasmic side.
  • Database links
  • Alternative names
    • FMN antibody
    • Formin (limb deformity) antibody
    • Formin 1 antibody
    • LD antibody
    • Limb deformity protein homolog antibody
    see all

Images

  • All lanes : Anti-FMN1 antibody (ab68058) at 1/500 dilution

    Lane 1 : FMN1 transfected 293T cell lysate
    Lane 2 : Non-transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

    Observed band size : 85 kDa (why is the actual band size different from the predicted?)

References

This product has been referenced in:
  • Gao Y  et al. Perfluorooctanesulfonate (PFOS)-induced Sertoli cell injury through a disruption of F-actin and microtubule organization is mediated by Akt1/2. Sci Rep 7:1110 (2017). WB, ICC/IF ; Rat . Read more (PubMed: 28439067) »
  • Li N  et al. Overexpression of plastin 3 in Sertoli cells disrupts actin microfilament bundle homeostasis and perturbs the tight junction barrier. Spermatogenesis 6:e1206353 (2016). Read more (PubMed: 27559491) »

See all 5 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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