Overview

  • Product nameAnti-FMN1 antibody
  • Description
    Mouse polyclonal to FMN1
  • SpecificityThis antibody is reactive against mammalian transfected lysate.
  • Tested applicationsSuitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    FMN1 (AAI03693.1, 1 a.a. ~ 503 a.a) full-length human protein.

  • Positive control
    • FMN1 transfected 293T cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab68058 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 85 kDa.
ICC/IF Use at an assay dependent concentration.

Target

  • RelevanceFMN1 plays a role in the formation of adherens junction and the polymerization of linear actin cables. Interacts with alpha-catenin and may interact with tubulin
  • Cellular localizationNucleus. Cytoplasm. Cell junction, adherens junction; Peripheral membrane protein; Cytoplasmic side.
  • Database links
  • Alternative names
    • FMN antibody
    • Formin (limb deformity) antibody
    • Formin 1 antibody
    • LD antibody
    • Limb deformity protein homolog antibody
    see all

Anti-FMN1 antibody images

  • All lanes : Anti-FMN1 antibody (ab68058) at 1/500 dilution

    Lane 1 : FMN1 transfected 293T cell lysate
    Lane 2 : Non-transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

    Observed band size : 85 kDa (why is the actual band size different from the predicted?)
  • ab68058 staining FMN1 in mouse embryonic fibroblast cells by Immunocytochemistry/ Immunofluorescence. The cells were fixed using 4% paraformaldehyde, permeabilised in 1% Triton X-100 and then blocked using 10% horse serum + 5% BSA for 1 hour at room temperature. Samples were then incubated with primary antibody at 1/500 for 16 hours at 4°C. The secondary antibody used was a goat anti-rabbit IgG conjugated to Alexa Fluor® 488 (green) used at a 1/500 dilution. DAPI was used to stain the cell nuclei (blue).

    See Abreview

References for Anti-FMN1 antibody (ab68058)

This product has been referenced in:
  • Lynch J  et al. Metastasis Suppressor microRNA-335 Targets the Formin Family of Actin Nucleators. PLoS One 8:e78428 (2013). WB ; Human . Read more (PubMed: 24223803) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"