Overview

  • Product nameAnti-FMN2 antibody
    See all FMN2 primary antibodies
  • Description
    Rabbit polyclonal to FMN2
  • Tested applicationsSuitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide (Human) corresponding to a region between residues 650-700 of human FMN2. (NP_064450.3).

  • Positive control
    • human brain lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab72052 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
ICC/IF
  • Application notesWB: Use at a concentration of 0.25 - 0.5 µg/ml. Detects bands of approximately 67 and 150 kDa (predicted molecular weight: 180 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionRequired for asymmetric spindle positioning, asymmetric oocyte division and polar body extrusion during female germ cell meiosis (By similarity). Actin-binding protein that is involved in actin cytoskeleton assembly and reorganization. Acts as an actin nucleation factor and promotes assembly of actin filaments together with SPIRE1 and SPIRE2. Involved in intracellular vesicle transport along actin fibers, providing a novel link between actin cytoskeleton dynamics and intracellular transport. Plays a role in responses to DNA damage, cellular stress and hypoxia by protecting CDKN1A against degradation, and thereby plays a role in stress-induced cell cycle arrest. Protects cells against apoptosis by protecting CDKN1A against degradation.
    • Tissue specificityExpressed almost exclusively in the developing and mature central nervous system.
    • Sequence similaritiesBelongs to the formin homology family. Cappuccino subfamily.
      Contains 1 FH1 (formin homology 1) domain.
      Contains 1 FH2 (formin homology 2) domain.
    • Cellular localizationCytoplasm > cytoskeleton. Cytoplasm > perinuclear region. Cytoplasm > cytosol. Cell membrane. Cytoplasmic vesicle membrane. Cytoplasm > cell cortex. Nucleus > nucleolus. Recruited to the membranes via its interaction with SPIRE1. Colocalizes with the actin cytoskeleton. Detected at the cleavage furrow during asymmetric oocyte division and polar body extrusion.
    • Information by UniProt
    • Database links
    • Alternative names
      • FMN 2 antibody
      • Fmn2 antibody
      • FMN2_HUMAN antibody
      • Formin 2 antibody
      • Formin-2 antibody
      see all

    Anti-FMN2 antibody images

    • All lanes : Anti-FMN2 antibody (ab72052) at 0.25 µg/ml

      Lane 1 : human brain lysate
      Lane 2 : human brain lysate with immunising peptide


      Predicted band size : 180 kDa
      Observed band size : 150 kDa (why is the actual band size different from the predicted?)
      Additional bands at : 67 kDa. We are unsure as to the identity of these extra bands.
    • ab72052 staining FMN2 in Mouse MEF cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with formaldehyde, permeabilzed with 1% Triton X-100 and blocked with 10% horse serum + 5% BSA for 1 hour at room temperature. Samples were incubated with primary antibody (1/500 in blocking buffer) for 16 hours at 4°C. An Alexa Fluor®-conjugated Goat anti-rabbit IgG polyclonal (1/500) was used as the secondary antibody.
      Left-hand panel: FMN2 staining in green.
      Centre-hand panel: DAPI staining in blue.
      Right-hand panel: Merged

      See Abreview

    References for Anti-FMN2 antibody (ab72052)

    This product has been referenced in:
    • Yu XJ  et al. The subcortical maternal complex controls symmetric division of mouse zygotes by regulating F-actin dynamics. Nat Commun 5:4887 (2014). Read more (PubMed: 25208553) »
    • Lynch J  et al. Metastasis Suppressor microRNA-335 Targets the Formin Family of Actin Nucleators. PLoS One 8:e78428 (2013). WB ; Human . Read more (PubMed: 24223803) »

    See all 2 Publications for this product

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