The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesWB: Use at a concentration of 0.25 - 0.5 µg/ml. Detects bands of approximately 67 and 150 kDa (predicted molecular weight: 180 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionRequired for asymmetric spindle positioning, asymmetric oocyte division and polar body extrusion during female germ cell meiosis (By similarity). Actin-binding protein that is involved in actin cytoskeleton assembly and reorganization. Acts as an actin nucleation factor and promotes assembly of actin filaments together with SPIRE1 and SPIRE2. Involved in intracellular vesicle transport along actin fibers, providing a novel link between actin cytoskeleton dynamics and intracellular transport. Plays a role in responses to DNA damage, cellular stress and hypoxia by protecting CDKN1A against degradation, and thereby plays a role in stress-induced cell cycle arrest. Protects cells against apoptosis by protecting CDKN1A against degradation.
Tissue specificityExpressed almost exclusively in the developing and mature central nervous system.
Cellular localizationCytoplasm > cytoskeleton. Cytoplasm > perinuclear region. Cytoplasm > cytosol. Cell membrane. Cytoplasmic vesicle membrane. Cytoplasm > cell cortex. Nucleus > nucleolus. Recruited to the membranes via its interaction with SPIRE1. Colocalizes with the actin cytoskeleton. Detected at the cleavage furrow during asymmetric oocyte division and polar body extrusion.
Immunocytochemistry/ Immunofluorescence - FMN2 antibody (ab72052)This image is courtesy of an anonymous Abreview
ab72052 staining FMN2 in Mouse MEF cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with formaldehyde, permeabilzed with 1% Triton X-100 and blocked with 10% horse serum + 5% BSA for 1 hour at room temperature. Samples were incubated with primary antibody (1/500 in blocking buffer) for 16 hours at 4°C. An Alexa Fluor®-conjugated Goat anti-rabbit IgG polyclonal (1/500) was used as the secondary antibody. Left-hand panel: FMN2 staining in green. Centre-hand panel: DAPI staining in blue. Right-hand panel: Merged