Anti-FMNL1 antibody (ab97456)
- Datasheet
- Specific References (2)
- Protocols
Overview
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Product nameAnti-FMNL1 antibody
See all FMNL1 primary antibodies -
DescriptionRabbit polyclonal to FMNL1
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Host speciesRabbit
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Tested applicationsSuitable for: WBmore details
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Species reactivityReacts with: Rat, Human
Predicted to work with: Mouse
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Immunogen
Recombinant protein fragment corresponding to a region within amino acids 631 - 873 of Human FMNL1 (NP_005883).
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Positive control
- 293T, A431, HepG2, MOLT4 and Raji cell lysates. H1299 and HeLa whole cell lysate.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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Storage bufferPreservative: 0.01% Thimerosal (merthiolate)
Constituents: 10% Glycerol, 0.1M Tris, 0.1M Glycine, pH 7.0 -
Concentration information loading... -
PurityImmunogen affinity purified
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab97456 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500 - 1/3000. Predicted molecular weight: 122 kDa. |
Target
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FunctionMay play a role in the control of cell motility and survival of macrophages (By similarity). Plays a role in the regulation of cell morphology and cytoskeletal organization. Required in the cortical actin filament dynamics and cell shape.
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Tissue specificityExpressed in heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas.
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Sequence similaritiesBelongs to the formin homology family.
Contains 1 DAD (diaphanous autoregulatory) domain.
Contains 1 FH2 (formin homology 2) domain.
Contains 1 GBD/FH3 (Rho GTPase-binding/formin homology 3) domain. -
DomainThe DAD domain regulates activation via by an autoinhibitory interaction with the N-terminus. This autoinhibition is released upon competitive binding of an activated GTPase. The release of DAD allows the FH2 domain to then nucleate and elongate nonbranched actin filaments.
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Post-translational
modificationsMyristoylation mediates membrane localization and blebbing. -
Cellular localizationCytoplasm > cell cortex. Cell projection > bleb. Colocalized with F-actin in bleb protrusions and Cytoplasm. Cell membrane. Cytoplasmic vesicle > phagosome. Recruited to actin-rich phagosomes during phagocytosis. Translocates to the plasma membrane upon activation by RAC1.
- Information by UniProt
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Database links
- Entrez Gene: 752 Human
- Entrez Gene: 57778 Mouse
- Entrez Gene: 287746 Rat
- Omim: 604656 Human
- SwissProt: O95466 Human
- SwissProt: Q9JL26 Mouse
- Unigene: 100217 Human
- Unigene: 138913 Mouse
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Alternative names
- 8030453N10Rik antibody
- AI553564 antibody
- C17orf1 antibody
see all
Images
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Western blot - Anti-FMNL1 antibody (ab97456)All lanes : Anti-FMNL1 antibody (ab97456) at 1/1000 dilution
Lane 1 : H1299 whole cell lysate
Lane 2 : HeLa whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 122 kDa
7.5% SDS PAGE
Protocols
References
This product has been referenced in:
- Kage F et al. FMNL formins boost lamellipodial force generation. Nat Commun 8:14832 (2017). Read more (PubMed: 28327544) »
- Monzo P et al. Mechanical confinement triggers glioma linear migration dependent on formin FHOD3. Mol Biol Cell 27:1246-61 (2016). Rat . Read more (PubMed: 26912794) »
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"