Anti-FMNL1 antibody (ab97456)

Overview

• Product name
  Anti-FMNL1 antibody
  See all FMNL1 primary antibodies
• Description
  Rabbit polyclonal to FMNL1
• Tested applications
  Suitable for: WBmore details
• Species reactivity
  Reacts with: Rat, Human
  Predicted to work with: Mouse
• Immunogen

  Recombinant protein fragment corresponding to a region within amino acids 631 - 873 of Human FMNL1 (NP_005883).

• Positive control
  • 293T, A431, HepG2, MOLT4 and Raji cell lysates. H1299 and HeLa whole cell lysate.

Properties

• Form
  Liquid
• Storage instructions
  Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
• Storage buffer
  Preservative: 0.01% Thimerosal (merthiolate)
  Constituents: 10% Glycerol, 0.1M Tris, 0.1M Glycine, pH 7.0
• Concentration information loading...
• Purity
  Immunogen affinity purified
• Clonality
  Polyclonal
• Isotype
  IgG
• Research areas
  • Immunology
  • Innate Immunity
  • Macrophage / Inflamm.

  • Cancer
  • Tumor biomarkers
  • Tumor antigens

Associated products

• Compatible Secondaries
  • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
  • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
• Positive Controls
  • HepG2 cytoplasmic extract lysate (ab14659)
  • HeLa whole cell lysate (ab29545)

Applications

Target

• Function
  May play a role in the control of cell motility and survival of macrophages (By similarity). Plays a role in the regulation of cell morphology and cytoskeletal organization. Required in the cortical actin filament dynamics and cell shape.
• Tissue specificity
  Expressed in heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas.
• Sequence similarities
  Belongs to the formin homology family.
  Contains 1 DAD (diaphanous autoregulatory) domain.
  Contains 1 FH2 (formin homology 2) domain.
  Contains 1 GBD/FH3 (Rho GTPase-binding/formin homology 3) domain.
• Domain
  The DAD domain regulates activation via by an autoinhibitory interaction with the N-terminus. This autoinhibition is released upon competitive binding of an activated GTPase. The release of DAD allows the FH2 domain to then nucleate and elongate nonbranched actin filaments.
• Post-translational
  modifications
  Myristoylation mediates membrane localization and blebbing.
• Cellular localization
  Cytoplasm > cell cortex. Cell projection > bleb. Colocalized with F-actin in bleb protrusions and Cytoplasm. Cell membrane. Cytoplasmic vesicle > phagosome. Recruited to actin-rich phagosomes during phagocytosis. Translocates to the plasma membrane upon activation by RAC1.

• Target information above from: UniProt accession O95466 The UniProt Consortium
  The Universal Protein Resource (UniProt) in 2010
  Nucleic Acids Res. 38:D142-D148 (2010) .

  Information by UniProt
• Database links
  • Entrez Gene: 752 Human
  • Entrez Gene: 57778 Mouse
  • Entrez Gene: 287746 Rat
  • Omim: 604656 Human
  • SwissProt: O95466 Human
  • SwissProt: Q9JL26 Mouse
  • Unigene: 100217 Human
  • Unigene: 138913 Mouse
• Alternative names
  • 8030453N10Rik antibody
  • AI553564 antibody
  • C17orf1 antibody

  • C17orf1B antibody
  • CLL associated antigen KW 13 antibody
  • CLL-associated antigen KW-13 antibody
  • FHOD4 antibody
  • FMNL antibody
  • FMNL_HUMAN antibody
  • Fmnl1 antibody
  • Fnrl antibody
  • Formin like 1 antibody
  • Formin like antibody
  • Formin related protein antibody
  • Formin-like protein 1 antibody
  • Frls antibody
  • KW 13 antibody
  • KW13 antibody
  • Leukocyte formin antibody
  • MGC133052 antibody
  • MGC1894 antibody
  • MGC21878 antibody

  see all

Protocols

• Western blot protocols