Overview

  • Product name
  • Description
    Rabbit polyclonal to FMO3
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Monkey
  • Immunogen

    Recombinant fragment corresponding to a region within amino acids 270-519 of Human FMO3 (UniProt ID: P31513).

  • Positive control
    • 293T, A431, H1299, HeLa, Molt4, Raji and mouse liver whole cell lysates; A431 cells; OVCAR3 xenograft tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab154089 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 60 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/100 - 1/1000.

Target

  • Function
    Involved in the oxidative metabolism of a variety of xenobiotics such as drugs and pesticides. It N-oxygenates primary aliphatic alkylamines as well as secondary and tertiary amines. Plays an important role in the metabolism of trimethylamine (TMA), via the production of TMA N-oxide (TMAO). Is also able to perform S-oxidation when acting on sulfide compounds.
  • Tissue specificity
    Liver.
  • Involvement in disease
    Defects in FMO3 are the cause of trimethylaminuria (TMAU) [MIM:602079]; also known as fish-odor syndrome. TMAU is an inborn error of metabolism associated with an offensive body odor and caused by deficiency of FMO-mediated N-oxidation of amino-trimethylamine (TMA) derived from foodstuffs. Such individuals excrete relatively large amounts of TMA in their urine, sweat, and breath, and exhibit a fishy body odor characteristic of the malodorous free amine.
  • Sequence similarities
    Belongs to the FMO family.
  • Cellular localization
    Microsome membrane. Endoplasmic reticulum membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Dimethylaniline monooxygenase [N oxide forming] 3 antibody
    • Dimethylaniline monooxygenase [N-oxide-forming] 3 antibody
    • Dimethylaniline monooxygenase 3 antibody
    • Dimethylaniline oxidase 3 antibody
    • dJ127D3.1 antibody
    • Flavin containing monooxygenase 3 antibody
    • FMO 3 antibody
    • FMO form 2 antibody
    • FMO II antibody
    • FMO3 antibody
    • FMO3_HUMAN antibody
    • FMOII antibody
    • Hepatic flavin containing monooxygenase 3 antibody
    • Hepatic flavin-containing monooxygenase 3 antibody
    • MGC34400 antibody
    • TMAU antibody
    • Trimethylamine monooxygenase antibody
    see all

Images

  • Anti-FMO3 antibody (ab154089) at 1/1000 dilution + H1299 whole cell lysate at 30 µg

    Predicted band size: 60 kDa



    7.5% SDS PAGE
  • Anti-FMO3 antibody (ab154089) at 1/1000 dilution + mouse liver whole cell lysate at 50 µg

    Predicted band size: 60 kDa



    7.5% SDS PAGE
  • Immunohistochemical analysis of paraffin-embedded OVCAR3 xenograft tissue labeling FMO3 with ab154089 at 1/100 dilution.
  • Immunofluorescence analysis of methanol-fixed A431 cells labeling FMO3 with ab154089 at 1/200 dilution. The image in the lower panel is costained with Hoechst 33342.

References

ab154089 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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