Overview

  • Product name
    Anti-FMO3 antibody [EPR6967]
    See all FMO3 primary antibodies
  • Description
    Rabbit monoclonal [EPR6967] to FMO3
  • Tested applications
    Suitable for: Flow Cyt, WBmore details
    Unsuitable for: IHC-P or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    A synthetic peptide corresponding to residues in Human FMO3 (UniProt P31513).

  • Positive control
    • Rat kidney, Mouse liver, Rat liver and Human fetal liver lysates.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Applications

Our Abpromise guarantee covers the use of ab126790 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/65. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
WB 1/1000 - 1/10000. Detects a band of approximately 56 kDa (predicted molecular weight: 60 kDa).
  • Application notes
    Is unsuitable for IHC-P or IP.
  • Target

    • Function
      Involved in the oxidative metabolism of a variety of xenobiotics such as drugs and pesticides. It N-oxygenates primary aliphatic alkylamines as well as secondary and tertiary amines. Plays an important role in the metabolism of trimethylamine (TMA), via the production of TMA N-oxide (TMAO). Is also able to perform S-oxidation when acting on sulfide compounds.
    • Tissue specificity
      Liver.
    • Involvement in disease
      Defects in FMO3 are the cause of trimethylaminuria (TMAU) [MIM:602079]; also known as fish-odor syndrome. TMAU is an inborn error of metabolism associated with an offensive body odor and caused by deficiency of FMO-mediated N-oxidation of amino-trimethylamine (TMA) derived from foodstuffs. Such individuals excrete relatively large amounts of TMA in their urine, sweat, and breath, and exhibit a fishy body odor characteristic of the malodorous free amine.
    • Sequence similarities
      Belongs to the FMO family.
    • Cellular localization
      Microsome membrane. Endoplasmic reticulum membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • Dimethylaniline monooxygenase [N oxide forming] 3 antibody
      • Dimethylaniline monooxygenase [N-oxide-forming] 3 antibody
      • Dimethylaniline monooxygenase 3 antibody
      • Dimethylaniline oxidase 3 antibody
      • dJ127D3.1 antibody
      • Flavin containing monooxygenase 3 antibody
      • FMO 3 antibody
      • FMO form 2 antibody
      • FMO II antibody
      • FMO3 antibody
      • FMO3_HUMAN antibody
      • FMOII antibody
      • Hepatic flavin containing monooxygenase 3 antibody
      • Hepatic flavin-containing monooxygenase 3 antibody
      • MGC34400 antibody
      • TMAU antibody
      • Trimethylamine monooxygenase antibody
      see all

    Images

    • Overlay histogram showing HepG2 cells stained with ab126790 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab126790, 1/65 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
    • All lanes : Anti-FMO3 antibody [EPR6967] (ab126790) at 1/1000 dilution

      Lane 1 : Rat kidney lysate
      Lane 2 : Mouse liver lysate
      Lane 3 : Rat liver lysate
      Lane 4 : Human fetal liver lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 60 kDa

    References

    This product has been referenced in:
    • Bennett BJ  et al. Trimethylamine-N-oxide, a metabolite associated with atherosclerosis, exhibits complex genetic and dietary regulation. Cell Metab 17:49-60 (2013). Read more (PubMed: 23312283) »

    See 1 Publication for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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