Recombinant
RabMAb

Anti-FMO3 antibody [EPR6968] (ab126711)

Overview

  • Product name
    Anti-FMO3 antibody [EPR6968]
    See all FMO3 primary antibodies
  • Description
    Rabbit monoclonal [EPR6968] to FMO3
  • Tested applications
    Suitable for: ICC/IF, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human FMO3 aa 300-400.
    Database link: P31513

  • Positive control
    • WB: Human fetal liver, human fetal kidney, mouse kidney, mouse liver, rat kidney and rat liver tissue lysates. IHC-P: Human, mouse and rat liver tissues. ICC/IF: HepG2 cells.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated ‘PUR’ on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Applications

Our Abpromise guarantee covers the use of ab126711 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/150.
WB 1/5000. Detects a band of approximately 56 kDa (predicted molecular weight: 60 kDa).

For unpurified use at 1/1000 - 1/10000.

IHC-P 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

See protocols (link: http://www.abcam.com/protocols/ihc-antigen-retrieval-protocol).

For unpurified use at 1/500 - 1/1000.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Target

  • Function
    Involved in the oxidative metabolism of a variety of xenobiotics such as drugs and pesticides. It N-oxygenates primary aliphatic alkylamines as well as secondary and tertiary amines. Plays an important role in the metabolism of trimethylamine (TMA), via the production of TMA N-oxide (TMAO). Is also able to perform S-oxidation when acting on sulfide compounds.
  • Tissue specificity
    Liver.
  • Involvement in disease
    Defects in FMO3 are the cause of trimethylaminuria (TMAU) [MIM:602079]; also known as fish-odor syndrome. TMAU is an inborn error of metabolism associated with an offensive body odor and caused by deficiency of FMO-mediated N-oxidation of amino-trimethylamine (TMA) derived from foodstuffs. Such individuals excrete relatively large amounts of TMA in their urine, sweat, and breath, and exhibit a fishy body odor characteristic of the malodorous free amine.
  • Sequence similarities
    Belongs to the FMO family.
  • Cellular localization
    Microsome membrane. Endoplasmic reticulum membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Dimethylaniline monooxygenase [N oxide forming] 3 antibody
    • Dimethylaniline monooxygenase [N-oxide-forming] 3 antibody
    • Dimethylaniline monooxygenase 3 antibody
    • Dimethylaniline oxidase 3 antibody
    • dJ127D3.1 antibody
    • Flavin containing monooxygenase 3 antibody
    • FMO 3 antibody
    • FMO form 2 antibody
    • FMO II antibody
    • FMO3 antibody
    • FMO3_HUMAN antibody
    • FMOII antibody
    • Hepatic flavin containing monooxygenase 3 antibody
    • Hepatic flavin-containing monooxygenase 3 antibody
    • MGC34400 antibody
    • TMAU antibody
    • Trimethylamine monooxygenase antibody
    see all

Images

  • All lanes : Anti-FMO3 antibody [EPR6968] (ab126711) at 1/5000 dilution (purified)

    Lane 1 : Human fetal kidney tissue lysate
    Lane 2 : Human fetal liver tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size : 60 kDa
    Observed band size : 56 kDa (why is the actual band size different from the predicted?)

    Blocking and diluting buffer: 5% NFDM/TBST.

  • All lanes : Anti-FMO3 antibody [EPR6968] (ab126711) at 1/5000 dilution (purified)

    Lane 1 : Mouse kidney tissue lysate
    Lane 2 : Mouse liver tissue lysate
    Lane 3 : Rat kidney tissue lysate
    Lane 4 : Rat liver tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size : 60 kDa
    Observed band size : 56 kDa (why is the actual band size different from the predicted?)

    Blocking and diluting buffer: 5% NFDM/TBST.

  • All lanes : Anti-FMO3 antibody [EPR6968] (ab126711) at 1/1000 dilution (unpurified)

    Lane 1 : Human fetal liver tissue lysate
    Lane 2 : Human fetal kidney tissue lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    HRP-conjugated goat anti-rabbit IgG at 1/2000 dilution

    Predicted band size : 60 kDa
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human liver tissue labelling FMO3 with purified ab126711 at 1/500. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse liver tissue labelling FMO3 with purified ab126711 at 1/500. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat liver tissue labelling FMO3 with purified ab126711 at 1/500. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

  • Immunohistochemitsy (Formalin/PFA-fixed paraffin-embedded sections) analysis of human liver tissue labelling FMO3 with unpurified ab126711 at a 1/500 dilution.

  • Immunocytochemistry/Immunofluorescence analysis of HepG2 cells labelling FMO3 with purified ab126711 at 1/150. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/500) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain. ab7291, a mouse anti-tubulin (1/500) and ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/500) were also used.

    Control 1: primary antibody (1/100) and secondary antibody, ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/500).

    Control 2: ab7291 (1/1000) and secondary antibody, ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/500).

  • Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD

References

ab126711 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Huh7)
Permeabilization
No
Specification
Huh7
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 22°C
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

Submitted Dec 08 2016

Application
Western blot
Sample
Mouse Cell lysate - whole cell (primary hepatoctyes)
Gel Running Conditions
Non-reduced Denaturing (8%)
Loading amount
30 µg
Specification
primary hepatoctyes
Blocking step
I-Block(Applied biosystems) as blocking agent for 30 mins as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2µg/mL · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Nov 16 2016

Application
Western blot
Loading amount
65 µg
Gel Running Conditions
Reduced Denaturing (Novex 4-12% Bis-Tris midi-gel)
Sample
Mouse Tissue lysate - whole (liver)
Specification
liver
Treatment
500ug/kg TCDD for 6hrs
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Abcam user community

Verified customer

Submitted Mar 10 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up