Overview

  • Product name
  • Description
    Rabbit polyclonal to FMR1NB
  • Tested applications
    Suitable for: ICC/IF, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    antigen sequence: VLANGHILPN SENAHGQSLE EDSALEALLN FFFPTTCNLR ENQVAKPCNE LQDLSESECL RHKCCFSSSG TTSFKCFAPF RDVPKQMMQM, corresponding to amino acids 98-187 of Human FMR1NB.

  • Positive control
    • Human Testis tissue; FMR1NB over-expression HEK293T lysate.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab121339 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 - 4 µg/ml.
IHC-P 1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/250 - 1/500.

Target

  • Tissue specificity
    Testis-specific. Expressed in melanoma, sarcoma, lung, breast, bladder, esophageal and ovarian cancers.
  • Sequence similarities
    Contains 1 P-type (trefoil) domain.
  • Cellular localization
    Membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Cancer/testis antigen 37 antibody
    • CT37 antibody
    • FLJ25736 antibody
    • FMR1N_HUMAN antibody
    • FMR1NB antibody
    • Fragile X mental retardation 1 neighbor antibody
    • Fragile X mental retardation 1 neighbor protein antibody
    • NY SAR 35 antibody
    • NYSAR35 antibody
    • Sarcoma antigen NY-SAR-35 antibody
    see all

Images

  • ab121339, at 1/50 dilution, staining FMR1NB in Paraffin-embedded Human Testis tissue by Immunohistochemistry.
  • All lanes : Anti-FMR1NB antibody (ab121339) at 1/250 dilution

    Lane 1 : Negative control (vector only transfected HEK293T lysate)
    Lane 2 : Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells

    Developed using the ECL technique
  • Immunofluorescent staining of Human cell line U-2 OS shows positivity in nucleoli using ab121339.
    Recommended treatment:
    Fixation/Permeabilization: PFA/Triton X-100

References

ab121339 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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