Overview

  • Product name
  • Description
    Rabbit polyclonal to FMRP
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Xenopus tropicalis
  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 85-260 of Human FMRP (UniProt: Q06787).

  • Positive control
    • HepG2 whole cell lysate; HeLa cells; Human DLD1 xenograft tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab154039 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 71 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/100 - 1/1000.

Target

  • Function
    Translation repressor. Component of the CYFIP1-EIF4E-FMR1 complex which binds to the mRNA cap and mediates translational repression. In the CYFIP1-EIF4E-FMR1 complex this subunit mediates translation repression (By similarity). RNA-binding protein that plays a role in intracellular RNA transport and in the regulation of translation of target mRNAs. Associated with polysomes. May play a role in the transport of mRNA from the nucleus to the cytoplasm. Binds strongly to poly(G), binds moderately to poly(U) but shows very little binding to poly(A) or poly(C).
  • Tissue specificity
    Highest levels found in neurons, brain, testis, placenta and lymphocytes. Also expressed in epithelial tissues and at very low levels in glial cells.
  • Involvement in disease
    Defects in FMR1 are the cause of fragile X syndrome (FRAX) [MIM:300624]. Fragile X syndrome is a common genetic disease (has a prevalence of one in every 2000 children) which is characterized by moderate to severe mental retardation, macroorchidism (enlargement of the testicles), large ears, prominent jaw, and high-pitched, jocular speech. The defect in most fragile X syndrome patients results from an amplification of a CGG repeat region which is directly in front of the coding region.
    Defects in FMR1 are the cause of fragile X tremor/ataxia syndrome (FXTAS) [MIM:300623]. In FXTAS, the expanded repeats range in size from 55 to 200 repeats and are referred to as 'premutations'. Full repeat expansions with greater than 200 repeats results in fragile X mental retardation syndrome [MIM:300624]. Carriers of the premutation typically do not show the full fragile X syndrome phenotype, but comprise a subgroup that may have some physical features of fragile X syndrome or mild cognitive and emotional problems.
    Defects in FMR1 are the cause of premature ovarian failure syndrome type 1 (POF1) [MIM:311360]. An ovarian disorder defined as the cessation of ovarian function under the age of 40 years. It is characterized by oligomenorrhea or amenorrhea, in the presence of elevated levels of serum gonadotropins and low estradiol.
  • Sequence similarities
    Belongs to the FMR1 family.
    Contains 2 KH domains.
  • Post-translational
    modifications
    Phosphorylated on several serine residues.
  • Cellular localization
    Cytoplasm. Nucleus > nucleolus.
  • Information by UniProt
  • Database links
  • Alternative names
    • FMR 1 antibody
    • Fmr1 antibody
    • Fmr1 gene antibody
    • FMR1_HUMAN antibody
    • FMRP antibody
    • Fragile X mental retardation 1 antibody
    • Fragile X mental retardation 1 protein antibody
    • Fragile X mental retardation protein 1 antibody
    • Fragile X mental retardation protein antibody
    • fragile X mental retardation syndrome-related protein 1 antibody
    • fragile X mental retardation, autosomal homolog 1 antibody
    • FRAXA antibody
    • fxr1 antibody
    • MGC87458 antibody
    • POF antibody
    • POF1 antibody
    • Protein FMR-1 antibody
    • Protein FMR1 antibody
    • wu:fb16f11 antibody
    • wu:fd18c10 antibody
    • zgc:66226 antibody
    see all

Images

  • Anti-FMRP antibody (ab154039) at 1/1000 dilution + HepG2 at 30 µg

    Predicted band size: 71 kDa



    10% SDS PAGE
  • Confocal immunofluorescence analysis of paraformaldehyde-fixed HeLa cells, labeling FMRP with ab154039 at 1/500 dilution. Alpha-tubulin filaments are labeled red.
  • Immunohistochemical analysis of paraffin-embedded Human DLD1 xenograft tissue, labeling FMRP with ab154039 at 1/100 dilution.

References

ab154039 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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