Overview

  • Product nameAnti-FMRP antibody
    See all FMRP primary antibodies
  • Description
    Rabbit polyclonal to FMRP
  • Tested applicationsSuitable for: IHC-P, WB, IHC-FoFr, ICC/IF, IPmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
    Predicted to work with: Orangutan
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 550 to the C-terminus of Human FMRP.

    (Peptide available as ab19074.)

  • Positive control
    • This antibody gave a positive signal in the following lysates: HeLa Whole Cell, HeLa Nuclear, Mouse Brain Tissue, PC12 whole cell.
  • General notesab27455 does not recognise endogenous FMRP (expected size 71 kDa) in human testes lysate, which may be due to low expression levels of FMRP.

Properties

Applications

Our Abpromise guarantee covers the use of ab17722 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 80 kDa (predicted molecular weight: 71 kDa).
IHC-FoFr Use at an assay dependent concentration.
ICC/IF Use a concentration of 5 µg/ml.
IP Use at an assay dependent concentration.

Target

  • FunctionTranslation repressor. Component of the CYFIP1-EIF4E-FMR1 complex which binds to the mRNA cap and mediates translational repression. In the CYFIP1-EIF4E-FMR1 complex this subunit mediates translation repression (By similarity). RNA-binding protein that plays a role in intracellular RNA transport and in the regulation of translation of target mRNAs. Associated with polysomes. May play a role in the transport of mRNA from the nucleus to the cytoplasm. Binds strongly to poly(G), binds moderately to poly(U) but shows very little binding to poly(A) or poly(C).
  • Tissue specificityHighest levels found in neurons, brain, testis, placenta and lymphocytes. Also expressed in epithelial tissues and at very low levels in glial cells.
  • Involvement in diseaseDefects in FMR1 are the cause of fragile X syndrome (FRAX) [MIM:300624]. Fragile X syndrome is a common genetic disease (has a prevalence of one in every 2000 children) which is characterized by moderate to severe mental retardation, macroorchidism (enlargement of the testicles), large ears, prominent jaw, and high-pitched, jocular speech. The defect in most fragile X syndrome patients results from an amplification of a CGG repeat region which is directly in front of the coding region.
    Defects in FMR1 are the cause of fragile X tremor/ataxia syndrome (FXTAS) [MIM:300623]. In FXTAS, the expanded repeats range in size from 55 to 200 repeats and are referred to as 'premutations'. Full repeat expansions with greater than 200 repeats results in fragile X mental retardation syndrome [MIM:300624]. Carriers of the premutation typically do not show the full fragile X syndrome phenotype, but comprise a subgroup that may have some physical features of fragile X syndrome or mild cognitive and emotional problems.
    Defects in FMR1 are the cause of premature ovarian failure syndrome type 1 (POF1) [MIM:311360]. An ovarian disorder defined as the cessation of ovarian function under the age of 40 years. It is characterized by oligomenorrhea or amenorrhea, in the presence of elevated levels of serum gonadotropins and low estradiol.
  • Sequence similaritiesBelongs to the FMR1 family.
    Contains 2 KH domains.
  • Post-translational
    modifications
    Phosphorylated on several serine residues.
  • Cellular localizationCytoplasm. Nucleus > nucleolus.
  • Information by UniProt
  • Database links
  • Alternative names
    • FMR 1 antibody
    • Fmr1 antibody
    • Fmr1 gene antibody
    • FMR1_HUMAN antibody
    • FMRP antibody
    • Fragile X mental retardation 1 antibody
    • Fragile X mental retardation 1 protein antibody
    • Fragile X mental retardation protein 1 antibody
    • Fragile X mental retardation protein antibody
    • fragile X mental retardation syndrome-related protein 1 antibody
    • fragile X mental retardation, autosomal homolog 1 antibody
    • FRAXA antibody
    • fxr1 antibody
    • MGC87458 antibody
    • POF antibody
    • POF1 antibody
    • Protein FMR-1 antibody
    • Protein FMR1 antibody
    • wu:fb16f11 antibody
    • wu:fd18c10 antibody
    • zgc:66226 antibody
    see all

Anti-FMRP antibody images

  • ab17722 staining FMRP in SK-N-SH cells treated with (R,S)-3,5-DHPG (ab120020), by ICC/IF. Increase in FMRP expression correlates with increased concentration of (R,S)-3,5-DHPG, as described in literature.
    The cells were incubated at 37°C for 1h in media containing different concentrations of ab120020 ((R,S)-3,5-DHPG) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab17722 (5 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.
  • IHC image of FMRP staining in mouse frontal cortex section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6). The section was then blocked using 1% BSA for 10 mins at 21°C The section was then incubated with ab17722,1/1500 , for 2 hours at 21°C. The section was then counterstained with haematoxylin.

    See Abreview

  • ICC/IF image of ab17722 stained HepG2 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab17722, 5µg/ml) overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti-rabbit IgG - H&L, pre-adsorbed (ab96899) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Anti-FMRP antibody (ab17722) at 1 µg/ml + Brain (Mouse) Tissue Lysate at 10 µg

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 71 kDa
    Observed band size : 80 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 105 kDa,23 kDa,75 kDa (possible isoform). We are unsure as to the identity of these extra bands.

    Exposure time : 4 minutes
  • ab17722 staining FMRP in SK-N-SH cells treated with (R,S)-MCPG sodium salt (ab120252), by ICC/IF. Decrease of FMRP expression correlates with increased concentration of (R,S)-MCPG sodium salt, as described in literature.
    The cells were incubated at 37°C for 2h in media containing different concentrations of ab120252 ((R,S)-MCPG sodium salt) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab17722 (5 μg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.
  • ab17722 staining FMRP in SK-N-SH cells treated with (S)-MCPG (ab120059), by ICC/IF. Decrease of FMRP expression correlates with increased concentration of (S)-MCPG, as described in literature.
    The cells were incubated at 37°C for 30 minutes in media containing different concentrations of ab120059 ((S)-MCPG) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab17722 (5 μg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.
  • ab17722 staining FMRP in SK-N-SH cells treated with (R,S)-MCPG (ab120033), by ICC/IF. Decrease in FMRP expression correlates with increased concentration of (R,S)-MCPG, as described in literature.
    The cells were incubated at 37°C for 2h in media containing different concentrations of ab120033 ((R,S)-MCPG) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab17722 (5 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.
  • All lanes : Anti-FMRP antibody (ab17722) at 1 µg/ml

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : HeLa (Human epithelial carcinoma cell line) Nuclear Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size : 71 kDa
    Observed band size : 80 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 75 kDa (possible isoform).
  • IHC image of FMRP staining in human tonsil FFPE section, performed on a BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab17722, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
  • ICC/IF image of ab17722 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab17722, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% PFA fixed (10 min) Hek293, HepG2 and MCF7 cells at 5µg/ml.
  • ab17722 staining FMRP in rat brain tissue section by Immunohistochemistry (PFA perfusion fixed frozen sections). Tissue from 4% PFA perfused animals underwent overnight fixation in 4% paraformaldehyde, cryoprotected in 30% sucrose and cut using cryostat.The primary antibody was diluted, 1/300 (PBS + 0.3% Triton X100) and incubated with sample for 18 hours at 20°C. An abcam antibody ab60314, Chromeo 488 conjugated goat polyclonal to rabbit IgG, diluted 1/1000 was used  as secondary.

    See Abreview

  • Anti-FMRP antibody (ab17722) at 1 µg/ml + PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 71 kDa
    Observed band size : 80 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 13 kDa,32 kDa,68 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 2 minutes
  • FMRP was immunoprecipitated using 0.5mg Hela whole cell extract, 5µg of Rabbit polyclonal to FMRP and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
    The antibody was incubated under agitation with Protein G beads for 10min, Hela whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
    Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab17722.
    Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).
    Band: 80kDa: FMRP.

References for Anti-FMRP antibody (ab17722)

This product has been referenced in:
  • Telias M  et al. Functional Deficiencies in Fragile X Neurons Derived from Human Embryonic Stem Cells. J Neurosci 35:15295-306 (2015). Read more (PubMed: 26586818) »
  • Taha MS  et al. Subcellular fractionation and localization studies reveal a direct interaction of the fragile X mental retardation protein (FMRP) with nucleolin. PLoS One 9:e91465 (2014). IP, IF ; Human . Read more (PubMed: 24658146) »

See all 15 Publications for this product

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 0.2% · Temperature: 25°C
Sample Mouse Cell (brain)
Specification brain
Permeabilization Yes - 0.1% TritonX-100
Fixative Formaldehyde
Username

Dr. Rubing Zhao-Shea

Verified customer

Submitted Aug 18 2014

Western blot

Excellent
Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Loading amount 10 µg
Gel Running Conditions Reduced Denaturing (10%)
Sample Human Cell lysate - whole cell (megakaryocytes)
Specification megakaryocytes
Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Username

Abcam user community

Verified customer

Submitted May 29 2013

Abreviews
Application Immunocytochemistry/ Immunofluorescence
Blocking step BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 21°C
Sample Chinese Hamster Cell (CHO cell line)
Specification CHO cell line
Permeabilization Yes - Triton-X 0.1%
Fixative Paraformaldehyde
Username

Dr. Loredana Bury

Verified customer

Submitted May 28 2013

Thank you for your reply and for kindly providing the further information and data.

Reviewing the details, I can suggest it would be important to consider the following:

Because there are so many isoforms of this protein, it is po...

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Thank you for taking the time to contact us. I am sorry to hear you have some concerns regarding the results from this antibody.

I would like to reassure you that this antibody is tested and covered by our 6 month guarantee for WB and in Mou...

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Thank you for contacting Abcam.


I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement.

To check the status of the or...

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Thank you for your call today and for letting us know about the trouble with ab17722.

As we discussed, please keep me updated about any new results with this antibody, and if the results are still not acceptable, I will be happy to send a dif...

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That's no problem. If you have any further questions please do not hesitate to ask.

Thank you for contacting us.

The anti-FMRP antibody (ab17722) has been raised against a synthetic peptide taken from within the residues 550 to the C-terminus of human FMRP. As can be seen from the sequence alignment with the chicken (attache...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Rat Tissue sections (brain: frontal cortex)
Specification brain: frontal cortex
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citric acid pH6
Permeabilization No
Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Username

Mr. Carl Hobbs

Verified customer

Submitted Sep 07 2010

1-10 of 14 Abreviews or Q&A

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