Overview

  • Product nameAnti-FN3K antibody
    See all FN3K primary antibodies
  • Description
    Mouse monoclonal to FN3K
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human, Recombinant Fragment
  • Immunogen

    Recombinant fragment, corresponding to amino acids 61-171 of Human FN3K with tag (NP_071441).

  • Positive control
    • HepG2 cell lysate; Recombinant fragment of human FNK3.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab77428 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 5 µg/ml. Detects a band of approximately 35 kDa (predicted molecular weight: 35 kDa).
ELISA Use at an assay dependent dilution.

Target

  • RelevanceFN3K catalyzes phosphorylation of fructosamines formed by glycation, the non-enzymatic reaction of glucose with primary amines followed by Amadori re-arrangement. Phosphorylation of fructosamines may initiate metabolism of the modified amine and lead to the de-glycation of fructoselysine and of glycated proteins.
  • Database links
  • Alternative names
    • 2310074G21Rik antibody
    • Fnsk antibody
    • Fructosamine 3 kinase antibody
    • RP23-293H17.4 antibody

Anti-FN3K antibody images

  • Anti-FN3K antibody (ab77428) at 5 µg/ml + Recombinant fragment of human FN3K at 0.2 µg

    Secondary
    Goat anti-Mouse IgG (H&L)-HRP at 1/5000 dilution

    Predicted band size : 35 kDa
    Observed band size : 38 kDa (why is the actual band size different from the predicted?)
  • Anti-FN3K antibody (ab77428) at 5 µg/ml + HepG2 cell lysate at 25 µg

    Secondary
    Goat anti-Mouse IgG (H&L)-HRP at 1/2500 dilution

    Predicted band size : 35 kDa
    Observed band size : 35 kDa

References for Anti-FN3K antibody (ab77428)

ab77428 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"