The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 - 5 µg/ml. Detects a band of approximately 35 kDa (predicted molecular weight: 35 kDa).
Use at an assay dependent dilution.
RelevanceFN3K catalyzes phosphorylation of fructosamines formed by glycation, the non-enzymatic reaction of glucose with primary amines followed by Amadori re-arrangement. Phosphorylation of fructosamines may initiate metabolism of the modified amine and lead to the de-glycation of fructoselysine and of glycated proteins.