Overview

  • Product nameAnti-FNBP3 antibody
    See all FNBP3 primary antibodies
  • Description
    Mouse polyclonal to FNBP3
  • Tested applicationsSuitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full-length Human protein (aa 1 - 215, AAH27178)

  • Positive control
    • WB: nuclear extracts from HeLa cells; ICC/IF: HeLa cells.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 1X PBS, pH 7.2
  • Concentration information loading...
  • PurityProtein A purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab88463 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
ICC/IF
  • Application notesICC/IF: Use at a concentration of 10 µg/ml.
    WB: Use at a concentration of 1 µg/ml. Predicted molecular weight for the unprocessed precursor: 109 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionBinds to WASL/N-WASP and suppresses its translocation from the nucleus to the cytoplasm, thereby inhibiting its cytoplasmic function (By similarity). May be involved in pre-mRNA splicing.
    • Tissue specificityExpressed in the brain cortex (at protein level). Widely expressed.
    • Sequence similaritiesBelongs to the PRPF40 family.
      Contains 5 FF domains.
      Contains 2 WW domains.
    • DomainThe WW domains are essential for localization to nuclear speckles.
    • Cellular localizationNucleus speckle. Nucleus matrix. Colocalizes with AKAP8L in the nuclear matrix.
    • Information by UniProt
    • Database links
    • Alternative names
      • Fas ligand associated factor 1 antibody
      • Fas ligand-associated factor 1 antibody
      • FBP 11 antibody
      • FBP11 antibody
      • FLAF 1 antibody
      • FLAF1 antibody
      • FNBP 3 antibody
      • Formin binding protein 11 antibody
      • Formin binding protein 3 antibody
      • Formin-binding protein 11 antibody
      • Formin-binding protein 3 antibody
      • HIP-10 antibody
      • HIP10 antibody
      • Huntingtin interacting protein A antibody
      • Huntingtin interacting protein HYPA antibody
      • Huntingtin yeast partner A antibody
      • Huntingtin-interacting protein 10 antibody
      • Huntingtin-interacting protein A antibody
      • HYPA antibody
      • NY REN 6 antibody
      • NY REN 6 antigen antibody
      • PR40A_HUMAN antibody
      • Pre mRNA processing factor 40 homolog A antibody
      • Pre-mRNA-processing factor 40 homolog A antibody
      • PRP40 pre mRNA processing factor 40 homolog A (S cerevisiae) antibody
      • PRP40 pre mRNA processing factor 40 homolog A (yeast) antibody
      • PRP40 pre mRNA processing factor 40 homolog A antibody
      • PRPF 40A antibody
      • PRPF40A antibody
      • Renal carcinoma antigen NY-REN-6 antibody
      see all

    Anti-FNBP3 antibody images

    • Anti-FNBP3 antibody (ab88463) at 1 µg/ml + HeLa nuclear extracts at 50 µg

      Predicted band size : 109 kDa
    • All lanes : Anti-FNBP3 antibody (ab88463) at 1 µg/ml

      Lane 1 : FNBP3 (AAH27178, aa 1-251)-transfected 293T cell lysate
      Lane 2 : non-transfected lysate

      Lysates/proteins at 25 µg per lane.


      Predicted band size : 109 kDa
    • Immunofluorescence staining of FNBP3 in HeLa cells using ab88463 at 10 µg/ml.

    References for Anti-FNBP3 antibody (ab88463)

    ab88463 has not yet been referenced specifically in any publications.

    Product Wall

    Thank you for contacting us.

    I am sorry that this antibody did not perform as stated on the datasheet. I have asked our accounting department to issue a credit note for you, which can be redeemed against the invoice of a future order by pass...

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    Thank you for forwarding the images of your blot.


    I agree that the band that you are detecting at the longer exposure does not seem to be specific. Except for the suggestions I had made previously, I am not sure that any other modificat...

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    Thank you for contacting Abcam regarding ab88463.


    I am sorry that you have been experiencing difficulties with this antibody in WB. I have reviewed the informatino you provided and wanted to clarify a few additional points.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"