Overview

  • Product nameAnti-FNDC3A antibody
    See all FNDC3A primary antibodies
  • Description
    Mouse polyclonal to FNDC3A
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length FNDC3A protein (Human), (ENSP00000347952, 1134 a.a).

  • Positive control
    • FNDC3A transfected 293T cell lysate and human placental tissue lysate.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: Whole serum
  • PurityWhole antiserum
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab68566 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
  • Application notesWB: 1/500 - 1/1000. Detects bands of approximately 120 and 200 kDa (predicted molecular weight: 125 kDa)

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • RelevanceFNDC3A is a member of a three-gene family in mice. It is expressed in several tissues including testis, and encodes a novel protein composed of a proline-rich amino-terminus, nine fibronectin type-III domains, and a hydrophobic carboxy-terminus. The proline-rich region of each family member contains conserved amino acids that include a PPGY consensus binding site for type I WW domain containing proteins. The hydrophobic carboxy-terminus is similar to that found in 'tail-anchored' proteins, integral membrane proteins that are localized to the cytosolic face of the endoplasmic reticulum. Immunohistochemical staining has shown that FNDC3A localizes to the acrosome of spermatids, as well as to Leydig cells in the mouse testis. FNDC3A may be involved in mediating spermatid-Sertoli adhesion during mouse spermatogenesis and mutation of FNDC3A is the cause of male sterility in sys mice. FNDC3A has also been found to be overexpressed in human odontoblasts.
    • Database links
    • Alternative names
      • Fibronectin type III domain containing protein 3a antibody
      • FNDC3 antibody
      • HUGO antibody
      • KIAA0970 antibody

    Anti-FNDC3A antibody images

    • Anti-FNDC3A antibody (ab68566) at 1/500 dilution + Human placental tissue lysate at 25 µg

      Secondary
      Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

      Predicted band size : 125 kDa
      Observed band size : 120 kDa (why is the actual band size different from the predicted?)
    • All lanes : Anti-FNDC3A antibody (ab68566) at 1/500 dilution

      Lane 1 : FNDC3A transfected 293T cell lysate
      Lane 2 : Non-transfected 293T cell lysate

      Lysates/proteins at 25 µg per lane.

      Secondary
      Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

      Predicted band size : 125 kDa
      Observed band size : 200 kDa (why is the actual band size different from the predicted?)

    References for Anti-FNDC3A antibody (ab68566)

    ab68566 has not yet been referenced specifically in any publications.

    Product Wall

    There are currently no Abreviews or Questions for ab68566.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"