Recombinant
RabMAb

Anti-FNDC5 antibody [EPR12209] (ab174833)

Overview

  • Product name
    Anti-FNDC5 antibody [EPR12209]
    See all FNDC5 primary antibodies
  • Description
    Rabbit monoclonal [EPR12209] to FNDC5
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WBmore details
    Unsuitable for: ICC/IF or IHC-P
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human FNDC5 aa 50-150 (Cysteine residue). The exact sequence is proprietary.
    Database link: Q8NAU1

  • Positive control
    • WB: Human fetal, mouse and rat muscle tissue lysates.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 40% Glycerol, 59% PBS, 0.05% BSA
  • Concentration information loading...
  • Purity
    Protein A purified
  • Clonality
    Monoclonal
  • Clone number
    EPR12209
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab174833 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Predicted molecular weight: 24 kDa.Can be blocked with FNDC5 peptide (ab204133).
  • Application notes
    Is unsuitable for ICC/IF or IHC-P.
  • Target

    Images

    • Anti-FNDC5 antibody [EPR12209] (ab174833) at 1/1000 dilution (purified) + Human fetal muscle tissue lysate at 10 µg

      Secondary
      HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG at 1/1000 dilution

      Predicted band size: 24 kDa
      Observed band size: 24 kDa



      Blocking buffer and concentration: 5% NFDM/TBST.

      Diluting buffer and concentration: 5% NFDM /TBST.

    • Anti-FNDC5 antibody [EPR12209] (ab174833) at 1/1000 dilution (purified) + Mouse muscle tissue lysate at 10 µg

      Secondary
      HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG at 1/1000 dilution

      Predicted band size: 24 kDa
      Observed band size: 24 kDa



      Blocking buffer and concentration: 5% NFDM/TBST.

      Diluting buffer and concentration: 5% NFDM /TBST.

    • Anti-FNDC5 antibody [EPR12209] (ab174833) at 1/5000 dilution (purified) + Rat muscle at 10 µg

      Secondary
      HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG at 1/1000 dilution

      Predicted band size: 24 kDa
      Observed band size: 24 kDa



      Blocking buffer and concentration: 5% NFDM/TBST.

      Diluting buffer and concentration: 5% NFDM /TBST.

    References

    This product has been referenced in:
    • Archundia-Herrera C  et al. Muscle irisin response to aerobic vs HIIT in overweight female adolescents. Diabetol Metab Syndr 9:101 (2017). Read more (PubMed: 29299068) »
    • Pérez-Sotelo D  et al. Lack of Adipocyte-Fndc5/Irisin Expression and Secretion Reduces Thermogenesis and Enhances Adipogenesis. Sci Rep 7:16289 (2017). Read more (PubMed: 29176631) »

    See all 5 Publications for this product

    Customer reviews and Q&As

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Mouse Cell lysate - whole cell (C2C12 cells)
    Gel Running Conditions
    Reduced Denaturing (11% SDS-PAGE)
    Loading amount
    30 µg
    Treatment
    2mM Metformin, 1mM AICAR, 1ng/ml TGF-beta1 for 24hr
    Specification
    C2C12 cells
    Blocking step
    BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    Submitted Jan 18 2017

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

    Sign up