Overview

  • Product name
  • Description
    Rabbit polyclonal to FNIP1
  • Tested applications
    Suitable for: WB, IPmore details
    Unsuitable for: IHC-P
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    A peptide at the N-terminus of FNIP1, MAPTLFQKLFSKRTGLGAC-amide

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.1% Sodium Azide
  • Purity
    Whole antiserum
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab61395 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/2000. Predicted molecular weight: 131 kDa.
IP Use at an assay dependent dilution.
  • Application notes
    Is unsuitable for IHC-P.
  • Target

    • Relevance
      May be involved in energy and/or nutrient sensing through the AMPK and mTOR signaling pathways. May regulate phosphorylation of RPS6KB1.
    • Cellular localization
      Cytoplasm. Note: Co-localizes with FLCN in the cytoplasm.
    • Database links
    • Alternative names
      • KIAA1961 antibody
      • FLCN-interacting protein antibody
      • FNIP1 antibody
      • Folliculin interacting protein 1 antibody

    References

    This product has been referenced in:
    • Siggs OM  et al. Mutation of Fnip1 is associated with B-cell deficiency, cardiomyopathy, and elevated AMPK activity. Proc Natl Acad Sci U S A 113:E3706-15 (2016). Read more (PubMed: 27303042) »
    • Liu J  et al. Coupling of mitochondrial function and skeletal muscle fiber type by a miR-499/Fnip1/AMPK circuit. EMBO Mol Med 8:1212-1228 (2016). Read more (PubMed: 27506764) »

    See all 2 Publications for this product

    Customer reviews and Q&As

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (HEK 293)
    Loading amount
    25 µg
    Specification
    HEK 293
    Gel Running Conditions
    Non-reduced Denaturing (7.5)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
    Username

    Abcam user community

    Verified customer

    Submitted Aug 22 2011

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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