Overview

  • Product nameAnti-FNTB antibody [EPR4706]
    See all FNTB primary antibodies
  • Description
    Rabbit monoclonal [EPR4706] to FNTB
  • Tested applicationsSuitable for: ICC/IF, WBmore details
    Unsuitable for: IHC-P
  • Species reactivity
    Reacts with: Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human FNTB aa 1-100 (N terminal).

  • Positive control
    • WB: JAR, HepG2, K562, SH-SY5Y and C6 cell lysates. ICC/IF: K562 cells.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated ‘PUR’ on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Properties

Applications

Our Abpromise guarantee covers the use of ab109748 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/500.

For unpurified use at 1/50 - 1/100.

WB 1/1000 - 1/10000. Predicted molecular weight: 49 kDa.
  • Application notesIs unsuitable for IHC-P.
  • Target

    • FunctionCatalyzes the transfer of a farnesyl moiety from farnesyl pyrophosphate to a cysteine at the fourth position from the C-terminus of several proteins. The beta subunit is responsible for peptide-binding.
    • Sequence similaritiesBelongs to the protein prenyltransferase subunit beta family.
      Contains 5 PFTB repeats.
    • Information by UniProt
    • Database links
    • Alternative names
      • CAAX farnesyltransferase subunit beta antibody
      • EC 2.5.1.58 antibody
      • EC=2.5.1.58 antibody
      • Farnesyltransferase antibody
      • farnesyltransferase CAAX box beta1 antibody
      • farnesyltransferase, CAAX box, beta antibody
      • FNTB antibody
      • FNTB_HUMAN antibody
      • FPTB antibody
      • FTase beta antibody
      • FTase-beta antibody
      • MGC105303 antibody
      • Protein farnesyltransferase subunit beta antibody
      • RAS proteins prenyltransferase beta antibody
      • Ras proteins prenyltransferase subunit beta antibody
      see all

    Anti-FNTB antibody [EPR4706] images

    • Anti-FNTB antibody [EPR4706] (ab109748) at 1/5000 dilution (purified) + C6 whole cell lysate at 10 µg

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

      Predicted band size : 49 kDa
      Observed band size : 46 kDa (why is the actual band size different from the predicted?)

      Blocking and dilution buffer: 5% NFDM/TBST.

    • All lanes : Anti-FNTB antibody [EPR4706] (ab109748) at 1/5000 dilution (purified)

      Lane 1 : JAR whole cell lysate
      Lane 2 : HepG2 whole cell lysate
      Lane 3 : K562 whole cell lysate
      Lane 4 : SH-SY5Y whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

      Predicted band size : 49 kDa
      Observed band size : 46 kDa (why is the actual band size different from the predicted?)

      Blocking and dilution buffer: 5% NFDM/TBST.

    • Immunocytochemistry/Immunofluorescence analysis of K562 cells labelling FNTB with purified ab109748 at 1/500. Cells were fixed with 100% methanol and permeabilized with 0.1% Triton X-100. ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain. ab7291, a mouse anti-tubulin (1/1000) and ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/1000) were also used.

      Control 1: primary antibody (1/500) and secondary antibody, ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/1000).

      Control 2: ab7291 (1/1000) and secondary antibody, ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000).

    • All lanes : Anti-FNTB antibody [EPR4706] (ab109748) at 1/1000 dilution (unpurified)

      Lane 1 : JAR cell lysate
      Lane 2 : HepG2 cell lysate
      Lane 3 : K562 cell lysate
      Lane 4 : SH-SY5Y cell lysate

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 49 kDa

    References for Anti-FNTB antibody [EPR4706] (ab109748)

    ab109748 has not yet been referenced specifically in any publications.

    Product Wall

    There are currently no Abreviews or Questions for ab109748.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"