Overview

  • Product nameAnti-FOG1 antibody
    See all FOG1 primary antibodies
  • Description
    Rabbit polyclonal to FOG1
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Cow
  • Immunogen

    Synthetic peptide corresponding to a region within the internal amino acids 755-804 (PPPPPGHAPA PESPRPGSGS GSGPGLAPAR SPGPAADGPI DLSKKPRRPL) of Human FOG1 (NP_722520).

  • Positive control
    • Fetal muscle lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab86281 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 104 kDa (predicted molecular weight: 104 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • FunctionTranscription regulator that plays an essential role in erythroid and megakaryocytic cell differentiation. Essential cofactor that acts via the formation of a heterodimer with transcription factors of the GATA family GATA1, GATA2 and GATA3. Such heterodimer can both activate or repress transcriptional activity, depending on the cell and promoter context. The heterodimer formed with GATA proteins is essential to activate expression of genes such as NFE2, ITGA2B, alpha- and beta-globin, while it represses expression of KLF1. May be involved in regulation of some genes in gonads. May also be involved in cardiac development, in a non-redundant way with ZFPM2/FOG2.
  • Tissue specificityMainly expressed in hematopoietic tissues. Also expressed in adult cerebellum, stomach, lymph node, liver and pancreas. Expressed in fetal heart, liver and spleen.
  • Sequence similaritiesBelongs to the FOG (Friend of GATA) family.
    Contains 4 C2H2-type zinc fingers.
    Contains 5 C2HC-type zinc fingers.
  • DomainThe CCHC-type zinc fingers 1, 5, 6 and 9 directly bind to GATA-type zinc fingers. The Tyr residue adjacent to the last Cys of the CCHC-type zinc finger is essential for the interaction with GATA-type zinc fingers.
  • Post-translational
    modifications
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • FOG-1 antibody
    • FOG1_HUMAN antibody
    • Friend of GATA 1 antibody
    • Friend of GATA protein 1 antibody
    • Friend of GATA1 antibody
    • ZFPM1 antibody
    • Zinc finger protein antibody
    • Zinc finger protein 89A antibody
    • Zinc finger protein multitype 1 antibody
    • Zinc finger protein ZFPM1 antibody
    see all

Anti-FOG1 antibody images

  • Anti-FOG1 antibody (ab86281) at 1 µg/ml (5% skim milk/ PBS buffer) + fetal muscle lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 104 kDa
    Observed band size : 104 kDa

References for Anti-FOG1 antibody (ab86281)

ab86281 has not yet been referenced specifically in any publications.

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