Anti-Folate Binding Protein antibody (ab67422)
- Datasheet
- Specific References (2)
- Protocols
Overview
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Product nameAnti-Folate Binding Protein antibody
See all Folate Binding Protein primary antibodies -
DescriptionRabbit polyclonal to Folate Binding Protein
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Host speciesRabbit
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Tested applicationsSuitable for: ICC/IF, WB, IHC-Pmore details
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Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat, Chicken
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Immunogen
Synthetic peptide conjugated to KLH derived from within residues 50 - 150 of Human Folate Binding Protein.
(Peptide available as ab87124.)
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Positive control
- This antibody gave a positive signal in Mouse Cerebellum Tissue Lysate. This antibody gave a positive result in IF in the following Formaldehyde fixed cell line: HepG2.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4 -
Concentration information loading... -
PurityImmunogen affinity purified
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
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Immunizing Peptide (Blocking)
Applications
Our Abpromise guarantee covers the use of ab67422 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | Use a concentration of 5 µg/ml. | |
| WB | Use a concentration of 1 µg/ml. Detects a band of approximately 28 kDa (predicted molecular weight: 30 kDa). Please note, we have been advised by some customers that ab67422 is unable to detect the human version of this protein in western blot. Please contact our scientific support services if you have any queries regarding this antibody. | |
| IHC-P | Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
Target
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FunctionBinds to folate and reduced folic acid derivatives and mediates delivery of 5-methyltetrahydrofolate to the interior of cells.
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Tissue specificityExclusively expressed in tissues of epithelial origin. Expression is increased in malignant tissues. Expressed in kidney, lung and cerebellum.
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Involvement in diseaseDefects in FOLR1 are the cause of neurodegeneration due to cerebral folate transport deficiency (NCFTD) [MIM:613068]. NCFTD is an autosomal recessive disorder resulting from brain-specific folate deficiency early in life. Onset is apparent in late infancy with severe developmental regression, movement disturbances, epilepsy, and leukodystrophy. Note=Recognition and diagnosis of this disorder is critical because folinic acid therapy can reverse the clinical symptoms and improve brain abnormalities and function.
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Sequence similaritiesBelongs to the folate receptor family.
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Post-translational
modificationsEight disulfide bonds are present.
The secreted form is derived from the membrane-bound form either by cleavage of the GPI anchor, or/and by proteolysis catalyzed by a metalloprotease. -
Cellular localizationCell membrane. Secreted.
- Information by UniProt
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Database links
- Entrez Gene: 2348 Human
- Entrez Gene: 14275 Mouse
- Entrez Gene: 171049 Rat
- Omim: 136430 Human
- SwissProt: P15328 Human
- SwissProt: P35846 Mouse
- Unigene: 73769 Human
- Unigene: 2135 Mouse
see all -
Alternative names
- adult antibody
- Adult folate binding protein antibody
- Adult folate-binding protein antibody
see all
Images
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Western blot - Anti-Folate Binding Protein antibody (ab67422)Anti-Folate Binding Protein antibody (ab67422) at 1/1 dilution + Cerebellum Mouse Tissue Lysate at 10 µg
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 30 kDa
Observed band size: 28 kDa (why is the actual band size different from the predicted?)
Additional bands at: 16 kDa (possible cleavage fragment), 22 kDa (possible cleavage fragment)
We hypothesize that the 28, 22 and 16 kDa bands represent the propeptide with signal sequence, propeptide without signal sequence and mature protein, respectively. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Folate Binding Protein antibody (ab67422)IHC image of Folate Binding Protein staining in human kidney carcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab67422, 10µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. -
Immunocytochemistry/ Immunofluorescence - Anti-Folate Binding Protein antibody (ab67422)ab67422 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab67422 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Protocols
References
This product has been referenced in:
- Siwowska K et al. Folate Receptor-Positive Gynecological Cancer Cells: In Vitro and In Vivo Characterization. Pharmaceuticals (Basel) 10:N/A (2017). Read more (PubMed: 28809784) »
- Ocak M et al. Folate receptor-targeted multimodality imaging of ovarian cancer in a novel syngeneic mouse model. Mol Pharm 12:542-53 (2015). Read more (PubMed: 25536192) »
Customer reviews and Q&As
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