Anti-Folate Binding Protein antibody (Biotin) (ab34529)

Overview

  • Product name
    Anti-Folate Binding Protein antibody (Biotin)
    See all Folate Binding Protein primary antibodies
  • Description
    Goat polyclonal to Folate Binding Protein (Biotin)
  • Conjugation
    Biotin
  • Tested applications
    Suitable for: WB, ELISA, Immunomicroscopy, Dot blotmore details
  • Species reactivity
    Reacts with: Cow
  • Immunogen

    Bovine Folate Binding Protein.

  • General notes

    10-20 Biotin molecules per goat IgG molecule.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 1% BSA, 0.42% Potassium phosphate, 0.87% Sodium chloride

    BSA is IgG and Protease free
  • Concentration information loading...
  • Purity
    IgG fraction
  • Purification notes
    This product is purified by a multi-step process including delipidation, salt fractionation, ion exchange chromatography and extensive dialysis against buffer.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab34529 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
ELISA
Immunomicroscopy
Dot blot
  • Application notes
    Dot: Use at an assay dependent dilution.
    ELISA: 1/4000 - 1/20000.
    This product has been assayed against 1.0µg of Folate Binding Protein in a standard capture ELISA using Peroxidase conjugated streptavidin and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic-acid]) as a substrate for 30 minutes at RT.
    IM: Use at an assay dependent dilution.
    WB: Use at an assay dependent dilution. Predicted molecular weight: 31 kDa.
    Suitable for other antibody based assays using streptavidin or avidin conjugates.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Binds to folate and reduced folic acid derivatives and mediates delivery of 5-methyltetrahydrofolate to the interior of cells.
    • Tissue specificity
      Exclusively expressed in tissues of epithelial origin. Expression is increased in malignant tissues. Expressed in kidney, lung and cerebellum.
    • Involvement in disease
      Defects in FOLR1 are the cause of neurodegeneration due to cerebral folate transport deficiency (NCFTD) [MIM:613068]. NCFTD is an autosomal recessive disorder resulting from brain-specific folate deficiency early in life. Onset is apparent in late infancy with severe developmental regression, movement disturbances, epilepsy, and leukodystrophy. Note=Recognition and diagnosis of this disorder is critical because folinic acid therapy can reverse the clinical symptoms and improve brain abnormalities and function.
    • Sequence similarities
      Belongs to the folate receptor family.
    • Post-translational
      modifications
      Eight disulfide bonds are present.
      The secreted form is derived from the membrane-bound form either by cleavage of the GPI anchor, or/and by proteolysis catalyzed by a metalloprotease.
    • Cellular localization
      Cell membrane. Secreted.
    • Information by UniProt
    • Database links
    • Alternative names
      • adult antibody
      • Adult folate binding protein antibody
      • Adult folate-binding protein antibody
      • FBP antibody
      • Folate Receptor 1 Adult antibody
      • Folate receptor 1 antibody
      • Folate Receptor 1 Precursor antibody
      • Folate receptor adult antibody
      • Folate receptor alpha antibody
      • Folate receptor antibody
      • FOLR antibody
      • FOLR1 antibody
      • FOLR1_HUMAN antibody
      • FR alpha antibody
      • FR-alpha antibody
      • FRalpha antibody
      • KB cells FBP antibody
      • MOV18 antibody
      • Ovarian cancer associated antigen antibody
      • Ovarian tumor associated antigen antibody
      • Ovarian tumor associated antigen MOv18 antibody
      • Ovarian tumor-associated antigen MOv18 antibody
      see all

    References

    ab34529 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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