Anti-Folate Binding Protein antibody [EPR4708(2)] (ab125030)

Overview

  • Product nameAnti-Folate Binding Protein antibody [EPR4708(2)]
    See all Folate Binding Protein primary antibodies
  • Description
    Rabbit monoclonal [EPR4708(2)] to Folate Binding Protein
  • Specificityab125030 does not recognize FOLR2.
  • Tested applicationsSuitable for: WBmore details
    Unsuitable for: ICC or IHC-P
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    corresponding to Human Folate Binding Protein aa 1-100.
    Database link: P15328

  • Positive control
    • JAR, HeLa, MCF7, 293T and A549 cell lysates
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated ‘PUR’ on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage bufferpH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 0.05% BSA, 40% Glycerol
  • Concentration information loading...
  • PurityProtein A purified
  • ClonalityMonoclonal
  • Clone numberEPR4708(2)
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab125030 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 30 kDa.
  • Application notesIs unsuitable for ICC or IHC-P.
  • Target

    • FunctionBinds to folate and reduced folic acid derivatives and mediates delivery of 5-methyltetrahydrofolate to the interior of cells.
    • Tissue specificityExclusively expressed in tissues of epithelial origin. Expression is increased in malignant tissues. Expressed in kidney, lung and cerebellum.
    • Involvement in diseaseDefects in FOLR1 are the cause of neurodegeneration due to cerebral folate transport deficiency (NCFTD) [MIM:613068]. NCFTD is an autosomal recessive disorder resulting from brain-specific folate deficiency early in life. Onset is apparent in late infancy with severe developmental regression, movement disturbances, epilepsy, and leukodystrophy. Note=Recognition and diagnosis of this disorder is critical because folinic acid therapy can reverse the clinical symptoms and improve brain abnormalities and function.
    • Sequence similaritiesBelongs to the folate receptor family.
    • Post-translational
      modifications
      Eight disulfide bonds are present.
      The secreted form is derived from the membrane-bound form either by cleavage of the GPI anchor, or/and by proteolysis catalyzed by a metalloprotease.
    • Cellular localizationCell membrane. Secreted.
    • Information by UniProt
    • Database links
    • Alternative names
      • adult antibody
      • Adult folate binding protein antibody
      • Adult folate-binding protein antibody
      • FBP antibody
      • Folate Receptor 1 Adult antibody
      • Folate receptor 1 antibody
      • Folate Receptor 1 Precursor antibody
      • Folate receptor adult antibody
      • Folate receptor alpha antibody
      • Folate receptor antibody
      • FOLR antibody
      • FOLR1 antibody
      • FOLR1_HUMAN antibody
      • FR alpha antibody
      • FR-alpha antibody
      • FRalpha antibody
      • KB cells FBP antibody
      • MOV18 antibody
      • Ovarian cancer associated antigen antibody
      • Ovarian tumor associated antigen antibody
      • Ovarian tumor associated antigen MOv18 antibody
      • Ovarian tumor-associated antigen MOv18 antibody
      see all

    Anti-Folate Binding Protein antibody [EPR4708(2)] images

    • All lanes : Anti-Folate Binding Protein antibody [EPR4708(2)] (ab125030) at 1/2000 dilution (purified)

      Lane 1 : mouse brain lysate
      Lane 2 : rat brain lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      HRP goat anti-rabbit (H+L) at 1/1000 dilution

      Predicted band size : 30 kDa
      Additional bands at : 37-42 kDa (possible glycosylated form).

      Blocking buffer: 5% NFDM/TBST

      Dilution buffer: 5% NFDM/TBST

    • Anti-Folate Binding Protein antibody [EPR4708(2)] (ab125030) at 1/2000 dilution (purified) + JAR cell lysate at 20 µg

      Secondary
      HRP goat ant-rabbit (H+L) at 1/1000 dilution

      Predicted band size : 30 kDa
      Additional bands at : 37-42 kDa (possible glycosylated form).

      Blocking buffer: 5% NFDM/TBST

      Dilution buffer: 5% NFDM/TBST

    • Lanes 1 - 2 : Anti-Folate Binding Protein antibody [EPR4708(2)] (ab125030) at 1/10000 dilution (purified)
      Lanes 3 - 4 : Anti-Folate Binding Protein antibody [EPR4708(2)] (ab125030) at 10000 (purified)

      Lane 1 : HeLa cell lysate
      Lane 2 : MCF-7 cell lysate
      Lane 3 : HEK293 cell lysate
      Lane 4 : A549 cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      Lanes 1 - 2 : HRP goat anti-rabbit (H+L) at 1/1000 dilution
      Lanes 3 - 4 : HRP goat anti-rabbit at 1/1000 dilution


      Predicted band size : 30 kDa
      Additional bands at : 37-42 kDa (possible glycosylated form).

      Blocking buffer: 5% NFDM/TBST

      Dilution buffer: 5% NFDM/TBST

    • All lanes : Anti-Folate Binding Protein antibody [EPR4708(2)] (ab125030) at 1/1000 dilution (Unpurified)

      Lane 1 : JAR cell lysate
      Lane 2 : HeLa cell lysate
      Lane 3 : MCF7 cell lysate
      Lane 4 : 293T cell lysate
      Lane 5 : A549 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP labelled goat anti rabbit at 1/2000 dilution

      Predicted band size : 30 kDa

    References for Anti-Folate Binding Protein antibody [EPR4708(2)] (ab125030)

    ab125030 has not yet been referenced specifically in any publications.

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Human Cell lysate - whole cell (HUES7 ES cells)
    Gel Running Conditions Reduced Denaturing (12%)
    Loading amount 20 µg
    Specification HUES7 ES cells
    Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 16°C
    Username

    Abcam user community

    Verified customer

    Submitted Feb 22 2016

    Application Western blot
    Sample Human Cell lysate - whole cell (HEPG2)
    Gel Running Conditions Reduced Denaturing (12.5)
    Loading amount 20 µg
    Specification HEPG2
    Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 16°C
    Username

    Abcam user community

    Verified customer

    Submitted Nov 13 2015

    Application Western blot
    Loading amount 100000 cells
    Gel Running Conditions Reduced Denaturing (10% gel)
    Sample Mouse Cell lysate - whole cell (mouse embryonic stem cell)
    Specification mouse embryonic stem cell
    Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 16°C
    Username

    Abcam user community

    Verified customer

    Submitted Aug 08 2014

    I think it is conceivable that there are differences between the hippocampus and liver forms and perhaps adult and pup. The UniProt database entry for human folate binding protein has these notes about expression and post-translation-modification.
    Read More

    Thank you for contacting us again and sending this new information.

    The WB image looks very convincing and proofs that the antibody is actually picking up its target as it should.

    Regarding the double band in the pup sample it may be ...

    Read More

    The 25 kDa band that you see is correct, given that the mature protein should be about 25 kDa, after removal of the signal peptide and propeptide from the precursor. (The "predicted" band size of 30 kDa is based on the size of the precursor of the prot...

    Read More

    Thank you for your reply.

    Unfortunately, it is quite hard to get some information if this protein expressed in the rat hippocampus. However, I checked the SwissProt site for more information regardingthis for the human protein, and it looks li...

    Read More

    Thank you for resending the image. Based on your description of the upper band as being ˜ 25kD it seems this could be the correct band, as the size can vary a few kD just based on the gel, buffer, etc. Adding a negative control could help ensure that t...

    Read More

    Thank you for your inquiry.

    Unfortunately, we did not test rat liver samples in WB from rat pups.

    We did test brain, heart, kidney and spleen of adult rat and see strong bands at ca. 38kDa in the brain and spleen samples. The calcul...

    Read More

    Thank you for confirming these details and for your cooperation.

    As requested, I have issued a free of charge replacement with ab125030, the order number is *******.

    To check the status of the order please contact our Customer Service...

    Read More

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