Anti-Folate Binding Protein antibody [LK26] (ab3361)

Overview

  • Product nameAnti-Folate Binding Protein antibody [LK26]
    See all Folate Binding Protein primary antibodies
  • Description
    Mouse monoclonal [LK26] to Folate Binding Protein
  • SpecificityWe have not conducted testing to determine whether the antibody is specific for the alpha or beta subtype of folate receptor.
  • Tested applicationsSuitable for: IHC-Fr, IP, Flow Cyt, ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Gestational choriocarcinoma cell line, Lu-75.

  • Positive control
    • Frozen Placenta or Ovarian Carcinoma (Negative control: Mouse serum or IgG1 isotype-matched negative control antibody, diluted to the same concentration as the primary antibody, should also be included in each run.)

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferConstituent: 1% BSA
  • Concentration information loading...
  • PurityIgG fraction
  • ClonalityMonoclonal
  • Clone numberLK26
  • IsotypeIgG2a
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab3361 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr 1/40.
IP Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration. PubMed: 19567824
ICC/IF Use at an assay dependent concentration. PubMed: 19636639
WB 1/500. PubMed: 18553957

Target

  • FunctionBinds to folate and reduced folic acid derivatives and mediates delivery of 5-methyltetrahydrofolate to the interior of cells.
  • Tissue specificityExclusively expressed in tissues of epithelial origin. Expression is increased in malignant tissues. Expressed in kidney, lung and cerebellum.
  • Involvement in diseaseDefects in FOLR1 are the cause of neurodegeneration due to cerebral folate transport deficiency (NCFTD) [MIM:613068]. NCFTD is an autosomal recessive disorder resulting from brain-specific folate deficiency early in life. Onset is apparent in late infancy with severe developmental regression, movement disturbances, epilepsy, and leukodystrophy. Note=Recognition and diagnosis of this disorder is critical because folinic acid therapy can reverse the clinical symptoms and improve brain abnormalities and function.
  • Sequence similaritiesBelongs to the folate receptor family.
  • Post-translational
    modifications
    Eight disulfide bonds are present.
    The secreted form is derived from the membrane-bound form either by cleavage of the GPI anchor, or/and by proteolysis catalyzed by a metalloprotease.
  • Cellular localizationCell membrane. Secreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • adult antibody
    • Adult folate binding protein antibody
    • Adult folate-binding protein antibody
    • FBP antibody
    • Folate Receptor 1 Adult antibody
    • Folate receptor 1 antibody
    • Folate Receptor 1 Precursor antibody
    • Folate receptor adult antibody
    • Folate receptor alpha antibody
    • Folate receptor antibody
    • FOLR antibody
    • FOLR1 antibody
    • FOLR1_HUMAN antibody
    • FR alpha antibody
    • FR-alpha antibody
    • FRalpha antibody
    • KB cells FBP antibody
    • MOV18 antibody
    • Ovarian cancer associated antigen antibody
    • Ovarian tumor associated antigen antibody
    • Ovarian tumor associated antigen MOv18 antibody
    • Ovarian tumor-associated antigen MOv18 antibody
    see all

Anti-Folate Binding Protein antibody [LK26] images

  • ab3361 staining frozen placenta in IHC-F.

References for Anti-Folate Binding Protein antibody [LK26] (ab3361)

This product has been referenced in:
  • Xie M  et al. Expression of folate receptors in nasopharyngeal and laryngeal carcinoma and folate receptor-mediated endocytosis by molecular targeted nanomedicine. Int J Nanomedicine 8:2443-51 (2013). Human . Read more (PubMed: 23874095) »
  • Roger E  et al. Folic Acid Functionalized Nanoparticles for Enhanced Oral Drug Delivery. Mol Pharm : (2012). Flow Cyt . Read more (PubMed: 22670575) »

See all 6 Publications for this product

Product Wall

It will depend on how you want to use the antibody and how much is required for each test . I updated the ab2107 datasheet to show suggested dilution ranges for the applications that are listed on the datasheet.

WB: 1/500 - 1/5000.
IP: ...

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Thank you for confirming these details and for your cooperation.

As requested, I have issued a free of charge replacement with ab125030, the order number is *******.

To check the status of the order please contact our Customer Service...

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Gracias por tu respuesta.

Si te parece puedo enviaros para que probéis un anticuerpo diferente contra la misma proteína.

Echarle un vistazo a los otros productos que tenemos disponibles contra Folate Binding Protein va...

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Muchas gracias por mandarnos el cuestionario relleno.

Para intentar optimizar los resultados del anticuerpo hay una serie de sugerencias que me gustaría hacer. De cualquier manera, al estar al anticuerpo bajo garantía en caso de...

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Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (THP-1)
Specification THP-1
Fixative Paraformaldehyde
Permeabilization No
Blocking step Protein Block (serum free) as blocking agent for 20 minute(s) · Concentration: 100% · Temperature: 4°C
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Abcam user community

Verified customer

Submitted Oct 13 2008

Thank you for your enquiry. Ab3361 was generated using gestational choriocarcinoma cell line, Lu-75, as the immunogen. However, we have not conducted testing to determine whether the antibody is specific for one or the other subtype of the receptor. ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"