Overview

Properties

Applications

Our Abpromise guarantee covers the use of ab58920 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 2.5 µg/ml. Detects a band of approximately 29 kDa (predicted molecular weight: 35 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

Images

  • Western blot - Anti-Follistatin antibody (ab58920)
    Western blot - Anti-Follistatin antibody (ab58920)
    Lane 1 : Anti-Follistatin antibody (ab58920) at 1 µg/ml
    Lane 2 : Anti-Follistatin antibody (ab58920) at 2.5 µg/ml
    Lane 3 : Anti-Follistatin antibody (ab58920) at 5 µg/ml

    All lanes : Cell lysate prepared from human Jurkat cells

    Lysates/proteins at 25 µg per lane.

    Predicted band size: 35 kDa

References

This product has been referenced in:
  • Jones AE  et al. Wnt/ß-catenin controls follistatin signalling to regulate satellite cell myogenic potential. Skelet Muscle 5:14 (2015). Read more (PubMed: 25949788) »
  • Gao MH  et al. beta(1)-Adrenergic receptor vs adenylyl cyclase 6 expression in cardiac myocytes: differences in transgene localization and intracellular signaling. Cell Signal 22:584-9 (2010). Read more (PubMed: 19932173) »

See all 3 Publications for this product

Publishing research using ab58920? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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6 reviews or Q&A

10
Votes: Highest First

Western blot abreview for Anti-Follistatin antibody

 Good
Abreviews
Application
Western blot
Sample
Sheep Tissue lysate - whole (Liver)
Gel Running Conditions
Reduced Denaturing (10% Tris Glycine Gels)
Loading amount
70 µg
Specification
Liver
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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Dr. John Odhiambo

Verified customer

Submitted Oct 22 2015

Thanks! We will order the smooth muscle actin antibody. While I was on your website looking for other potential antibodies, I also noted follistatin (ab58920)which is predicted to work with zebrafish, but there is not data available. Would it please be...

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DISCOUNT CODE:xxxxxxx
Expiration date: 20/03/2013
Value: CAD356.00
I am very pleased to offer you to test ab58920 in zebrafish. Because the only tested application for this product is WB it would be imprtant to tests the antibody on zebraf...

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Please arrange FOC of the same item.
Regards

Thank you for kindly confirming these details. I am sorry this vial of antibody has not worked for this customer.

As requested, we are pleased to arrange a free of charge replacement. I can confirm that 1 vial of ab58920 has been added to yo...

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Dear Kate,

Here is some quiries from customer:


Can you provide detail at what concentration i should use this antibody? they are asking to use 1:100-1:50 dilution and one vial contain only 100ul. if i will prepare 10ml of pr...

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Thank you for your message and for providing this further information.

I can confirm, as previously discussed, that the recommended concentration for Western blot using this antibody is 2.5 µg/ml (1:400) as stated on the datasheet. This is a...

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The antibody that we have now works beautifully; however, we were wondering if maybe the mouse antibody was more specific than the rabbit antibody. That was our only question.

Sorry for the misunderstanding. Both antibodies have been developed to recognise specifically the follistatin. I would say that both products are equally specific to the target. The polyclonal antibody was raised against a 49 amino acid l...

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Immunohistochemistry (Frozen sections) abreview for Anti-Follistatin antibody

 Poor
Abreviews
Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Embryonic mouse head E16)
Specification
Embryonic mouse head E16
Fixative
Paraformaldehyde
Permeabilization
Yes - Triton X-100 (0.1%)
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: RT°C
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Dr. Gaskon Ibarretxe

Verified customer

Submitted Feb 01 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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