- Datasheet
- Specific References (3)
- Protocols
Overview
- Product nameAnti-Follistatin antibody
See all Follistatin primary antibodies - DescriptionRabbit polyclonal to Follistatin
- Tested applicationsSuitable for: WBmore details
- Species reactivityReacts with: Rat, Human
Predicted to work with: Mouse, Sheep, Rabbit, Goat, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Pig, Zebrafish
- Immunogen
A region within synthetic peptide: LCDELCPDSK SDEPVCASDN ATYASECAMK EAACSSGVLL EVKHSGSCN, corresponding to N terminal amino acids 269-317 of Human Follistatin
- Positive control
- Purchase matching WB positive control:Recombinant Human Follistatin protein
- Jurkat cell lysate.
Properties
- FormLiquid
- Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
- Storage bufferPreservative: 0.09% Sodium azide
Constituents: 2% Sucrose, PBS -
Concentration information loading... - PurityProtein A purified
- ClonalityPolyclonal
- IsotypeIgG
- Research areas
Associated products
- Compatible Secondaries
- Positive Controls
Applications
Our Abpromise guarantee covers the use of ab58920 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use a concentration of 2.5 µg/ml. Detects a band of approximately 29 kDa (predicted molecular weight: 35 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T. |
Target
- FunctionBinds directly to activin and functions as an activin antagonist. Specific inhibitor of the biosynthesis and secretion of pituitary follicle stimulating hormone (FSH).
- Tissue specificityIsoform 1 is the predominant isoform in serum but is undetectable in follicular fluid.
- Sequence similaritiesContains 3 follistatin-like domains.
Contains 3 Kazal-like domains.
Contains 1 TB (TGF-beta binding) domain. - Cellular localizationSecreted.
- Information by UniProt
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Database links
- Entrez Gene: 396119 Chicken
- Entrez Gene: 327681 Cow
- Entrez Gene: 10468 Human
- Entrez Gene: 14313 Mouse
- Entrez Gene: 445002 Pig
- Entrez Gene: 24373 Rat
- Entrez Gene: 100004116 Zebrafish
- Omim: 136470 Human
see all -
Alternative names
- Activin binding protein antibody
- Activin-binding protein antibody
- Follistatin antibody
see all
Anti-Follistatin antibody images
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Western blot - Follistatin antibody (ab58920)Lane 1 : Anti-Follistatin antibody (ab58920) at 1 µg/ml
Lane 2 : Anti-Follistatin antibody (ab58920) at 2.5 µg/ml
Lane 3 : Anti-Follistatin antibody (ab58920) at 5 µg/ml
Lane 1 : Cell lysate prepared from human Jurkat cells
Lane 2 : Cell lysate prepared from human Jurkat cells
Lane 3 : Cell lysate prepared from human Jurkat cells
Lysates/proteins at 25 µg per lane.
Predicted band size : 35 kDa
References for Anti-Follistatin antibody (ab58920)
This product has been referenced in:
- Jones AE et al. Wnt/ß-catenin controls follistatin signalling to regulate satellite cell myogenic potential. Skelet Muscle 5:14 (2015). Read more (PubMed: 25949788) »
- Smith IJ et al. Sepsis downregulates myostatin mRNA levels without altering myostatin protein levels in skeletal muscle. J Cell Biochem 111:1059-73 (2010). WB ; Rat . Read more (PubMed: 20677217) »
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"