Overview

Properties

Applications

Our Abpromise guarantee covers the use of ab58920 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 2.5 µg/ml. Detects a band of approximately 29 kDa (predicted molecular weight: 35 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

Images

  • Lane 1 : Anti-Follistatin antibody (ab58920) at 1 µg/ml
    Lane 2 : Anti-Follistatin antibody (ab58920) at 2.5 µg/ml
    Lane 3 : Anti-Follistatin antibody (ab58920) at 5 µg/ml

    All lanes : Cell lysate prepared from human Jurkat cells

    Lysates/proteins at 25 µg per lane.

    Predicted band size: 35 kDa

References

This product has been referenced in:
  • Jones AE  et al. Wnt/ß-catenin controls follistatin signalling to regulate satellite cell myogenic potential. Skelet Muscle 5:14 (2015). Read more (PubMed: 25949788) »
  • Gao MH  et al. beta(1)-Adrenergic receptor vs adenylyl cyclase 6 expression in cardiac myocytes: differences in transgene localization and intracellular signaling. Cell Signal 22:584-9 (2010). Read more (PubMed: 19932173) »

See all 3 Publications for this product

Customer reviews and Q&As

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Application
Western blot
Sample
Sheep Tissue lysate - whole (Liver)
Gel Running Conditions
Reduced Denaturing (10% Tris Glycine Gels)
Loading amount
70 µg
Specification
Liver
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Dr. John Odhiambo

Verified customer

Submitted Oct 22 2015

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Embryonic mouse head E16)
Specification
Embryonic mouse head E16
Fixative
Paraformaldehyde
Permeabilization
Yes - Triton X-100 (0.1%)
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: RT°C
Username

Dr. Gaskon Ibarretxe

Verified customer

Submitted Feb 01 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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