Overview

Properties

Applications

Our Abpromise guarantee covers the use of ab64490 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 38 kDa (predicted molecular weight: 38 kDa).

Target

Anti-Follistatin antibody images

  • All lanes : Anti-Follistatin antibody (ab64490) at 1 µg/ml

    Lane 1 : U2OS (Human osteosarcoma cell line) Whole Cell Lysate
    Lane 2 : Liver (Mouse) Tissue Lysate
    Lane 3 : Raji (Human Burkitt's lymphoma cell line) Whole Cell Lysate
    Lane 4 : MOLT4 (Human acute lymphoblastic leukemia cell line) Whole Cell Lysate
    Lane 5 : Colon (Mouse) Tissue Lysate
    Lane 6 : Liver (Rat) Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 38 kDa
    Observed band size : 38 kDa
    Additional bands at : 15 kDa,75 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 1 minute

References for Anti-Follistatin antibody (ab64490)

This product has been referenced in:
  • Garcia LA  et al. 1,25(OH)2vitamin D3 stimulates myogenic differentiation by inhibiting cell proliferation and modulating the expression of promyogenic growth factors and myostatin in C2C12 skeletal muscle cells. Endocrinology 152:2976-86 (2011). WB ; Mouse . Read more (PubMed: 21673099) »

See 1 Publication for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Rat Tissue lysate - whole (muscle)
Loading amount 10 µg
Specification muscle
Gel Running Conditions Reduced Denaturing (9%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Dr. Chrispina Hong

Verified customer

Submitted Jan 18 2013

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (E10.5 mouse embryo sections)
Specification E10.5 mouse embryo sections
Fixative Paraformaldehyde
Permeabilization Yes - PBST (PBS / 0.5% v/v TritonX100 (Sigma))
Blocking step NOVOCASTRA protein block as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Oct 11 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Tissue lysate - whole (mouse distal embryo e7.5)
Loading amount 2 µg
Specification mouse distal embryo e7.5
Gel Running Conditions Reduced Denaturing (12% gel)
Blocking step Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 4°C
Username

Abcam user community

Verified customer

Submitted Aug 31 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"