Recombinant
RabMAb

Anti-Follistatin antibody [EPR10903] (ab157471)

Overview

  • Product name
    Anti-Follistatin antibody [EPR10903]
    See all Follistatin primary antibodies
  • Description
    Rabbit monoclonal [EPR10903] to Follistatin
  • Tested applications
    Suitable for: WBmore details
    Unsuitable for: Flow Cyt,ICC,IHC-P or IP
  • Species reactivity
    Reacts with: Rat, Human
  • Immunogen

    Recombinant fragment corresponding to a region within Human Follistatin (UniProt P19883).

  • Positive control
    • HepG2 and C6 cell lysates.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Mouse: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab157471 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Predicted molecular weight: 38 kDa.
  • Application notes
    Is unsuitable for Flow Cyt,ICC,IHC-P or IP.
  • Target

    • Function
      Binds directly to activin and functions as an activin antagonist. Specific inhibitor of the biosynthesis and secretion of pituitary follicle stimulating hormone (FSH).
    • Tissue specificity
      Isoform 1 is the predominant isoform in serum but is undetectable in follicular fluid.
    • Sequence similarities
      Contains 3 follistatin-like domains.
      Contains 3 Kazal-like domains.
      Contains 1 TB (TGF-beta binding) domain.
    • Cellular localization
      Secreted.
    • Information by UniProt
    • Database links
    • Alternative names
      • Activin binding protein antibody
      • Activin-binding protein antibody
      • Follistatin antibody
      • FS antibody
      • fst antibody
      • FST_HUMAN antibody
      • Human follistatin gene antibody
      see all

    Images

    • All lanes : Anti-Follistatin antibody [EPR10903] (ab157471) at 1/1000 dilution

      Lane 1 : HepG2 cell lysate
      Lane 2 : C6 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 38 kDa

    References

    This product has been referenced in:
    • Mock K  et al. The EMT-activator ZEB1 induces bone metastasis associated genes including BMP-inhibitors. Oncotarget 6:14399-412 (2015). WB . Read more (PubMed: 25973542) »

    See 1 Publication for this product

    Customer reviews and Q&As

    Application
    Western blot
    Sample
    Mouse Tissue lysate - whole (Mouse Ovary)
    Gel Running Conditions
    Reduced Denaturing (12)
    Loading amount
    40 µg
    Specification
    Mouse Ovary
    Blocking step
    Odyssey Blocking Buffer (Li-Cor) as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 26°C
    Username

    Abcam user community

    Verified customer

    Submitted May 11 2015

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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