- Datasheet
- Specific References
- Protocols
Overview
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Product nameAnti-FOLR2 antibody
See all FOLR2 primary antibodies -
DescriptionRabbit polyclonal to FOLR2
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Host speciesRabbit
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Tested applicationsSuitable for: ICC/IF, WBmore details
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Species reactivityReacts with: Human
Predicted to work with: Rat
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Immunogen
Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human FOLR2.
(Peptide available as ab104497.)
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Positive control
- This antibody gave a positive signal in the following human tissue lysates: Spleen; Bone marrow; Thymus
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4 -
Concentration information loading... -
PurityImmunogen affinity purified
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
Applications
Our Abpromise guarantee covers the use of ab103998 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | Use a concentration of 5 µg/ml. | |
| WB | Use a concentration of 1 µg/ml. Detects a band of approximately 35 kDa (predicted molecular weight: 32 kDa). |
Target
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FunctionBinds to folate and reduced folic acid derivatives and mediates delivery of 5-methyltetrahydrofolate to the interior of cells.
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Tissue specificityExpressed in placenta and hematopoietic cells. Expression is increased in malignant tissues.
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Sequence similaritiesBelongs to the folate receptor family.
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Post-translational
modificationsEight disulfide bonds are present.
N-glycosylated. -
Cellular localizationCell membrane. Secreted.
- Information by UniProt
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Database links
- Entrez Gene: 2350 Human
- Entrez Gene: 293154 Rat
- Omim: 136425 Human
- SwissProt: P14207 Human
- Unigene: 433159 Human
- Unigene: 8801 Rat
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Alternative names
- beta hFR antibody
- FBP antibody
- FBP/PL 1 antibody
see all
Images
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Western blot - Anti-FOLR2 antibody (ab103998)All lanes : Anti-FOLR2 antibody (ab103998) at 1 µg/ml
Lane 1 : Human spleen tissue lysate - total protein (ab29699)
Lane 2 : Bone marrow (Human) Tissue Lysate
Lane 3 : Human thymus tissue lysate - total protein (ab30146)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 32 kDa
Observed band size: 35 kDa (why is the actual band size different from the predicted?)
Exposure time: 8 minutes
FOLR2 contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted. -
Immunocytochemistry/ Immunofluorescence - Anti-FOLR2 antibody (ab103998)ICC/IF image of ab103998 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab103998 at 5µg/ml overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti- rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in Hek293, HepG2, and MCF-7 formaldehyde fixed cells at 5ug/ml and also in HeLa, Hek293, HepG2, and MCF-7 Methanol fixed cells at 5ug/ml.
References
ab103998 has not yet been referenced specifically in any publications.
Customer reviews and Q&As
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