Anti-FOLR2 antibody (ab103998)
Key features and details
- Rabbit polyclonal to FOLR2
- Suitable for: ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-FOLR2 antibody
See all FOLR2 primary antibodies -
Description
Rabbit polyclonal to FOLR2 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Rat -
Immunogen
Synthetic peptide corresponding to Human FOLR2 aa 1-100 conjugated to keyhole limpet haemocyanin.
(Peptide available asab104497) -
Positive control
- This antibody gave a positive signal in the following human tissue lysates: Spleen; Bone marrow; Thymus
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab103998 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF |
Use a concentration of 5 µg/ml.
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WB |
Use a concentration of 1 µg/ml. Detects a band of approximately 35 kDa (predicted molecular weight: 32 kDa).
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Notes |
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ICC/IF
Use a concentration of 5 µg/ml. |
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 35 kDa (predicted molecular weight: 32 kDa). |
Target
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Function
Binds to folate and reduced folic acid derivatives and mediates delivery of 5-methyltetrahydrofolate to the interior of cells. -
Tissue specificity
Expressed in placenta and hematopoietic cells. Expression is increased in malignant tissues. -
Sequence similarities
Belongs to the folate receptor family. -
Post-translational
modificationsEight disulfide bonds are present.
N-glycosylated. -
Cellular localization
Cell membrane. Secreted. - Information by UniProt
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Database links
- Entrez Gene: 2350 Human
- Entrez Gene: 293154 Rat
- Omim: 136425 Human
- SwissProt: P14207 Human
- Unigene: 433159 Human
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Alternative names
- beta hFR antibody
- FBP antibody
- FBP/PL 1 antibody
see all
Images
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All lanes : Anti-FOLR2 antibody (ab103998) at 1 µg/ml
Lane 1 : Human spleen tissue lysate - total protein (ab29699)
Lane 2 : Bone marrow (Human) Tissue Lysate
Lane 3 : Human thymus tissue lysate - total protein (ab30146)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 32 kDa
Observed band size: 35 kDa why is the actual band size different from the predicted?
Exposure time: 8 minutes
FOLR2 contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted. -
ICC/IF image of ab103998 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab103998 at 5µg/ml overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti- rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in Hek293, HepG2, and MCF-7 formaldehyde fixed cells at 5ug/ml and also in HeLa, Hek293, HepG2, and MCF-7 Methanol fixed cells at 5ug/ml.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab103998 has not yet been referenced specifically in any publications.