Overview

  • Product nameAnti-FOLR2 antibody
    See all FOLR2 primary antibodies
  • Description
    Rabbit polyclonal to FOLR2
  • Tested applicationsSuitable for: ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Rat
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human FOLR2.

    (Peptide available as ab104497.)

  • Positive control
    • This antibody gave a positive signal in the following human tissue lysates: Spleen; Bone marrow; Thymus

Properties

Applications

Our Abpromise guarantee covers the use of ab103998 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 35 kDa (predicted molecular weight: 32 kDa).

Target

  • FunctionBinds to folate and reduced folic acid derivatives and mediates delivery of 5-methyltetrahydrofolate to the interior of cells.
  • Tissue specificityExpressed in placenta and hematopoietic cells. Expression is increased in malignant tissues.
  • Sequence similaritiesBelongs to the folate receptor family.
  • Post-translational
    modifications
    Eight disulfide bonds are present.
    N-glycosylated.
  • Cellular localizationCell membrane. Secreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • beta hFR antibody
    • FBP antibody
    • FBP/PL 1 antibody
    • FBP2 antibody
    • fetal/placental antibody
    • folate receptor 2 (fetal) antibody
    • Folate receptor 2 antibody
    • Folate receptor antibody
    • Folate receptor beta antibody
    • Folate receptor, fetal/placental antibody
    • Folbp 2 antibody
    • Folbp2 antibody
    • Folr2 antibody
    • FOLR2_HUMAN antibody
    • FR beta antibody
    • FR P3 antibody
    • FR-beta antibody
    • Placental folate binding protein antibody
    • Placental folate-binding protein antibody
    see all

Anti-FOLR2 antibody images

  • All lanes : Anti-FOLR2 antibody (ab103998) at 1 µg/ml

    Lane 1 : Human spleen tissue lysate - total protein (ab29699)
    Lane 2 : Bone marrow (Human) Tissue Lysate
    Lane 3 : Human thymus tissue lysate - total protein (ab30146)

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 32 kDa
    Observed band size : 35 kDa (why is the actual band size different from the predicted?)


    Exposure time : 8 minutes
  • ICC/IF image of ab103998 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab103998 at 5µg/ml overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti- rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in Hek293, HepG2, and MCF-7 formaldehyde fixed cells at 5ug/ml and also in HeLa, Hek293, HepG2, and MCF-7 Methanol fixed cells at 5ug/ml.

References for Anti-FOLR2 antibody (ab103998)

ab103998 has not yet been referenced specifically in any publications.

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Expiration date: February 1, 2013

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"