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In Abcam's Formate Assay Kit, formate is oxidized to generate a product resulting in color formation (? = 450 nm) proportional to formate concentration. The kit provides a convenient means for detecting formate in biological samples such as in serum or plasma, cells, culture and fermentation media. There is no need for pretreatment or purification of samples
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Formic acid (HCOOH) is the simplest carboxylic acid and occurs naturally, notably in ant and bee venom. It is normally present at low levels in blood and urine (up to ~ 0.5 mM) but can be present in concentrations as high as 5 mM in acute methanol poisoning. Environmental exposure to formaldehyde can also elevate the blood and urine levels of formate.
|Formate Assay Buffer||WM||1 x 25ml|
|Formate Enzyme Mix - Lyophilized||Green cap||1 vial|
|Formate Standard (100 mM)||Yellow cap||1 x 100µl|
|Formate Substrate Mix- Lyophilized||Red cap||1 vial|
Our Abpromise guarantee covers the use of ab111748 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Functional Studies||Use at an assay dependent dilution.|
ab111748 has not yet been referenced specifically in any publications.