Overview

  • Product name
    Formate Assay Kit
  • Sample type
    Urine, Serum, Plasma, Other biological fluids, Tissue Extracts, Cell Lysate
  • Assay type
    Quantitative
  • Assay time
    1h 00m
  • Product overview

    In Abcam's Formate Assay Kit, formate is oxidized to generate a product resulting in color formation (? = 450 nm) proportional to formate concentration. The kit provides a convenient means for detecting formate in biological samples such as in serum or plasma, cells, culture and fermentation media. There is no need for pretreatment or purification of samples
    Visit our FAQs page for tips and troubleshooting.

  • Notes

    Formic acid (HCOOH) is the simplest carboxylic acid and occurs naturally, notably in ant and bee venom. It is normally present at low levels in blood and urine (up to ~ 0.5 mM) but can be present in concentrations as high as 5 mM in acute methanol poisoning. Environmental exposure to formaldehyde can also elevate the blood and urine levels of formate.

  • Tested applications
    Suitable for: Functional Studiesmore details

Properties

Applications

Our Abpromise guarantee covers the use of ab111748 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Functional Studies Use at an assay dependent dilution.

Images

  • Example of standard curve obtained using ab111748

Protocols

References

ab111748 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Thank you for contacting Abcam

The recipe for the Formate assay buffer is proprietary, but it does contain some NP-40 (< 0.5 %).

Please let me know if you have any other questions.

Thank you for your enquiry.

I agree that perhaps a mechanical extraction method would be best for this assay. Here is a link to a publication that discusses a variety of different methods to produce cell extracts from cyanobacteria.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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