Overview

  • Product nameAnti-Fos B antibody
    See all Fos B primary antibodies
  • Description
    Rabbit polyclonal to Fos B
  • Specificityab23469 reacts with fosB protein.
  • Tested applicationsSuitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Dog
  • Immunogen

    Synthetic peptide: SWLLPPPPPP PLPFQTSQDA PPNLTASLFT HSEVQVLGDP FPVVNPSYTS, corresponding to a region within C terminal amino acids 253-302 of Human Fos B

  • Positive control
    • HepG2 cell lysate and human lung.

Properties

Applications

Our Abpromise guarantee covers the use of ab23469 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1.25 µg/ml. Detects a band of approximately 36 kDa (predicted molecular weight: 36 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use at an assay dependent concentration.

Target

Anti-Fos B antibody images

  • Anti-Fos B antibody (ab23469) at 1.25 µg/ml + HepG2 cell lysate

    Secondary
    HRP conjugated anti-Rabbit IgG

    Performed under reducing conditions.

    Predicted band size : 36 kDa
    Observed band size : 36 kDa
  • Cells with positive label: Aveolar cells (indicated with arrows). Antibody concentration 4-8ug/ml. Magnification 400X.

References for Anti-Fos B antibody (ab23469)

ab23469 has not yet been referenced specifically in any publications.

Product Wall

Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Antigen retrieval step None
Sample Mouse Tissue sections (Brain)
Specification Brain
Permeabilization No
Fixative Paraformaldehyde
Username

Dr. Karine Thibault

Verified customer

Submitted Aug 22 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"