• Product nameAnti-FOXA1 antibody [SP88]
    See all FOXA1 primary antibodies
  • Description
    Rabbit monoclonal [SP88] to FOXA1
  • Tested applicationsSuitable for: ICC/IF, IHC-P, Flow Cytmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    A synthetic peptide derived from near C-terminus of Human FOXA1.

  • Positive control
    • Human Prostate This antibody gave a positive result when used in the following formaldehyde fixed cell lines: MCF-7.



Our Abpromise guarantee covers the use of ab99892 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 µg/ml.
IHC-P 1/100. Boil tissue section in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.
Flow Cyt Use at an assay dependent concentration. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.


  • FunctionTranscription factor that is involved in embryonic development, establishment of tissue-specific gene expression and regulation of gene expression in differentiated tissues. Is thought to act as a 'pioneer' factor opening the compacted chromatin for other proteins through interactions with nucleosomal core histones and thereby replacing linker histones at target enhancer and/or promoter sites. Binds DNA with the consensus sequence 5'-[AC]A[AT]T[AG]TT[GT][AG][CT]T[CT]-3' (By similarity). Proposed to play a role in translating the epigenetic signatures into cell type-specific enhancer-driven transcriptional programs. Its differential recruitment to chromatin is dependent on distribution of histone H3 methylated at 'Lys-5' (H3K4me2) in estrogen-regulated genes. Involved in the development of multiple endoderm-derived organ systems such as liver, pancreas, lung and prostate; FOXA1 and FOXA2 seem to have at least in part redundant roles (By similarity). Modulates the transcriptional activity of nuclear hormone receptors. Is involved in ESR1-mediated transcription; required for ESR1 binding to the NKX2-1 promoter in breast cancer cells; binds to the RPRM promter and is required for the estrogen-induced repression of RPRM. Involved in regulation of apoptosis by inhibiting the expression of BCL2. Involved in cell cycle regulation by activating expression of CDKN1B, alone or in conjunction with BRCA1. Originally discribed as a transcription activator for a number of liver genes such as AFP, albumin, tyrosine aminotransferase, PEPCK, etc. Interacts with the cis-acting regulatory regions of these genes. Involved in glucose homeostasis.
  • Tissue specificityHighly expressed in prostate and ESR1-positive breast tumors. Overexpressed in esophageal and lung adenocarcinomas.
  • Sequence similaritiesContains 1 fork-head DNA-binding domain.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • forkhead box A1 antibody
    • Forkhead box protein A1 antibody
    • FOX A1 antibody
    • FOXA1 antibody
    • FOXA1_HUMAN antibody
    • hepatocyte nuclear factor 3 alpha antibody
    • Hepatocyte nuclear factor 3-alpha antibody
    • HNF 3A antibody
    • HNF-3-alpha antibody
    • HNF-3A antibody
    • HNF3A antibody
    • MGC33105 antibody
    • TCF 3A antibody
    • TCF-3A antibody
    • TCF3A antibody
    • Transcription factor 3A antibody
    see all

Anti-FOXA1 antibody [SP88] images

  • Flow cytometric analysis of rabbit anti-FOXA1 (SP88) antibody ab99892 in HepG2 cells (green) compared to negative control of rabbit IgG (blue).

  • Staining of Human FOXA1 in a Formalin/PFA-fixed paraffin-embedded section of Human Prostate using ab99892 at a dilution of 1/100.
  • ab99892 stained MCF-7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab99892 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-FOXA1 antibody [SP88] (ab99892)

ab99892 has not yet been referenced specifically in any publications.

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