Anti-FOXA2 antibody (ab5074)
Key features and details
- Goat polyclonal to FOXA2
- Suitable for: WB
- Reacts with: Rat, Human
- Isotype: IgG
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Overview
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Product name
Anti-FOXA2 antibody
See all FOXA2 primary antibodies -
Description
Goat polyclonal to FOXA2 -
Host species
Goat -
Specificity
This antibody is expected to recognise both reported isoforms. -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Rat, Human
Predicted to work with: Chicken, Xenopus laevis -
Immunogen
Synthetic peptide: GVYSRPIMNSS, corresponding to amino acids 447-457 of Human FOXA 2.
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Positive control
- WB: Rat lung and human liver tissue lysates.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab5074 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 60 kDa (predicted molecular weight: 48 kDa).
A 1 hour primary incubation is recommended for this product. A nuclear extraction procedure may be required to concentrate the protein in samples. |
Notes |
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WB
Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 60 kDa (predicted molecular weight: 48 kDa). A 1 hour primary incubation is recommended for this product. A nuclear extraction procedure may be required to concentrate the protein in samples. |
Target
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Function
Transcription factor that is involved in embryonic development, establishment of tissue-specific gene expression and regulation of gene expression in differentiated tissues. Is thought to act as a 'pioneer' factor opening the compacted chromatin for other proteins through interactions with nucleosomal core histones and thereby replacing linker histones at target enhancer and/or promoter sites. Binds DNA with the consensus sequence 5'-[AC]A[AT]T[AG]TT[GT][AG][CT]T[CT]-3' (By similarity). In embryonic development is required for notochord formation. Involved in the development of multiple endoderm-derived organ systems such as the liver, pancreas and lungs; FOXA1 and FOXA2 seem to have at least in part redundant roles. Originally discribed as a transcription activator for a number of liver genes such as AFP, albumin, tyrosine aminotransferase, PEPCK, etc. Interacts with the cis-acting regulatory regions of these genes. Involved in glucose homeostasis; regulates the expression of genes important for glucose sensing in pancreatic beta-cells and glucose homeostasis. Involved in regulation of fat metabolism. Binds to fibrinogen beta promoter and is involved in IL6-induced fibrinogen beta transcriptional activation. -
Sequence similarities
Contains 1 fork-head DNA-binding domain. -
Post-translational
modificationsPhosphorylation on Thr-156 abolishes binding to target promoters and subsequent transcription activation upon insulin stimulation. -
Cellular localization
Nucleus. Cytoplasm. Shuttles between the nucleus and cytoplasm in a CRM1-dependent manner and in response to insulin signaling via AKT1 is exported from the nucleus. - Information by UniProt
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Database links
- Entrez Gene: 3170 Human
- Entrez Gene: 25099 Rat
- Omim: 600288 Human
- SwissProt: Q9Y261 Human
- SwissProt: P32182 Rat
- Unigene: 155651 Human
- Unigene: 10948 Rat
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Alternative names
- Forkhead box A2 antibody
- Forkhead box protein A2 antibody
- FOX A2 antibody
see all
Images
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Anti-FOXA2 antibody (ab5074) at 1 µg/ml + Rat Lung tissue lysate at 35 µg
Predicted band size: 48 kDa
Observed band size: 60 kDa why is the actual band size different from the predicted?Primary incubation was 1 hour. Detected by chemiluminescence.
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Anti-FOXA2 antibody (ab5074) at 1 µg/ml + Human Liver lysate at 1 µg/ml
Predicted band size: 48 kDa
Observed band size: 60 kDa why is the actual band size different from the predicted?Primary incubation was 1 hour. Detected by chemiluminescence.
Protocols
Datasheets and documents
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Datasheet download
References (11)
ab5074 has been referenced in 11 publications.
- Soda M et al. Repeated human deciduous tooth-derived dental pulp cell reprogramming factor transfection yields multipotent intermediate cells with enhanced iPS cell formation capability. Sci Rep 9:1490 (2019). PubMed: 30728386
- Shen J et al. Loss of FoxA2 accelerates neoplastic changes in the intrahepatic bile duct partly via the MAPK signaling pathway. Aging (Albany NY) 11:9280-9294 (2019). PubMed: 31689237
- Bhanu NV et al. Histone modification profiling reveals differential signatures associated with human embryonic stem cell self-renewal and differentiation. Proteomics 16:448-58 (2016). Flow Cyt ; Human . PubMed: 26631989
- Saitoh I et al. Choice of Feeders Is Important When First Establishing iPSCs Derived From Primarily Cultured Human Deciduous Tooth Dental Pulp Cells. Cell Med 8:9-23 (2015). PubMed: 26858904
- Maier JA et al. Foxa1 and Foxa2 are required for formation of the intervertebral discs. PLoS One 8:e55528 (2013). IHC . PubMed: 23383217