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Our Abpromise guarantee covers the use of ab60721 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|EMSA||Use at an assay dependent concentration. PubMed: 20650961|
|IP||Use at an assay dependent concentration.|
|WB||Use a concentration of 1 - 5 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 48 kDa).|
|ELISA||Use at an assay dependent concentration. The detection limit for recombinant tagged FOXA2 is approximately 0.03ng/ml as a capture antibody.|
|Flow Cyt||Use 0.5µg for 106 cells. ab170191-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.|
|ICC/IF||Use a concentration of 10 µg/ml.|
Immunoprecipitation analysis of FOXA2 transfected lysate using ab60721 and protein A magnetic bead