Anti-FOXA2 antibody [PR-3G1] (ab95327)

Overview

  • Product nameAnti-FOXA2 antibody [PR-3G1]
    See all FOXA2 primary antibodies
  • Description
    Mouse monoclonal [PR-3G1] to FOXA2
  • Specificityab95327 reacts with FOXA2. It does not react with other proteins from the FOX/ HNF family.
  • Tested applicationsSuitable for: WB, Indirect ELISAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant full length Human FOXA2 expressed in E. coli and refolded to soluble form.

  • Positive control
    • I-ELISA: Immobilized FOXA2 immunogen WB: HEK-293-FOXA2 transfectant cell lysate and FOXA2 recombinant protein
  • General notesThis product is for Research Use Only and not to be used for any commercial resale purpose, including, but not limited to, using the product as part of any developed assay technology such as ELISA, ELISPOT, or Multiplex assay that is sold to another end-user.

Properties

Applications

Our Abpromise guarantee covers the use of ab95327 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Predicted molecular weight: 48 kDa.
Indirect ELISA 1/1000.

Target

  • FunctionTranscription factor that is involved in embryonic development, establishment of tissue-specific gene expression and regulation of gene expression in differentiated tissues. Is thought to act as a 'pioneer' factor opening the compacted chromatin for other proteins through interactions with nucleosomal core histones and thereby replacing linker histones at target enhancer and/or promoter sites. Binds DNA with the consensus sequence 5'-[AC]A[AT]T[AG]TT[GT][AG][CT]T[CT]-3' (By similarity). In embryonic development is required for notochord formation. Involved in the development of multiple endoderm-derived organ systems such as the liver, pancreas and lungs; FOXA1 and FOXA2 seem to have at least in part redundant roles. Originally discribed as a transcription activator for a number of liver genes such as AFP, albumin, tyrosine aminotransferase, PEPCK, etc. Interacts with the cis-acting regulatory regions of these genes. Involved in glucose homeostasis; regulates the expression of genes important for glucose sensing in pancreatic beta-cells and glucose homeostasis. Involved in regulation of fat metabolism. Binds to fibrinogen beta promoter and is involved in IL6-induced fibrinogen beta transcriptional activation.
  • Sequence similaritiesContains 1 fork-head DNA-binding domain.
  • Post-translational
    modifications
    Phosphorylation on Thr-156 abolishes binding to target promoters and subsequent transcription activation upon insulin stimulation.
  • Cellular localizationNucleus. Cytoplasm. Shuttles between the nucleus and cytoplasm in a CRM1-dependent manner and in response to insulin signaling via AKT1 is exported from the nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Forkhead box A2 antibody
    • Forkhead box protein A2 antibody
    • FOX A2 antibody
    • foxa2 antibody
    • FOXA2_HUMAN antibody
    • Hepatic nuclear factor 3 beta antibody
    • Hepatocyte nuclear factor 3 antibody
    • Hepatocyte nuclear factor 3 beta antibody
    • Hepatocyte nuclear factor 3-beta antibody
    • HNF 3B antibody
    • HNF-3-beta antibody
    • HNF-3B antibody
    • HNF3B antibody
    • MGC19807 antibody
    • TCF 3B antibody
    • TCF-3B antibody
    • TCF3B antibody
    • Transcription factor 3B antibody
    see all

Anti-FOXA2 antibody [PR-3G1] images

  • All lanes : Anti-FOXA2 antibody [PR-3G1] (ab95327) at 1 µg/ml

    Lane 1 : HEK-293-FOXA2 transfectant cell lysate at 7.5 µg
    Lane 2 : Untransfected HEK-293 cell lysate at 7.5 µg
    Lane 3 : FOXA2 recombinant protein at 0.25 µg


    Predicted band size : 48 kDa
  • Indirect ELISA detection of immobilized FOXA2 immunogen with titrated amounts of ab95327. No binding is seen to other recombinant proteins in the FOX/ HNF family.

References for Anti-FOXA2 antibody [PR-3G1] (ab95327)

ab95327 has not yet been referenced specifically in any publications.

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Vielen Dank für Ihren Anruf.

Wie versprochen habe ich alle unsere FOXA2-Antikörper darauf geprüft, welche Isoformen von FOXA2 sie erkennen. Leider sind alle Immunogene in Regionen lokalisiert, die in beiden Isoformen vorkommen (Details siehe...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"