Recombinant Anti-FOXB2 antibody [EPR11476] (ab170897)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR11476] to FOXB2
- Suitable for: WB, IP
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-FOXB2 antibody [EPR11476]
See all FOXB2 primary antibodies -
Description
Rabbit monoclonal [EPR11476] to FOXB2 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details
Unsuitable for: Flow Cyt,ICC/IF or IHC-P -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- HeLa and LoVo cell lysates.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR11476 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab170897 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000 - 1/5000. Predicted molecular weight: 45 kDa.
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IP |
1/10 - 1/100.
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Notes |
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WB
1/1000 - 1/5000. Predicted molecular weight: 45 kDa. |
IP
1/10 - 1/100. |
Target
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Function
Transcription factor. -
Sequence similarities
Contains 1 fork-head DNA-binding domain. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 442425 Human
- Entrez Gene: 14240 Mouse
- Entrez Gene: 691398 Rat
- SwissProt: Q5VYV0 Human
- SwissProt: Q64733 Mouse
- Unigene: 553843 Human
- Unigene: 7971 Mouse
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Alternative names
- bA159H20.4 antibody
- Forkhead box B2 antibody
- Forkhead box protein B2 antibody
see all
Images
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Western blot analysis on immunoprecipitation pellet from HeLa cell lysate (Lane 1) or 1X PBS (negative control) (Lane 2) using ab170897 and HRP-conjugated anti-rabbit IgG which preferentially detects the non-reduced form of rabbit IgG.
Lane 1 Primary: ab170897, 1/1000 dilution
Sample: Immunoprecipitation pellet from HeLa cell lysate
Secondary: HRP-conjugated anti-rabbit IgGLane 2 Primary: ab170897, 1/1000 dilution
Sample: 1X PBS (negative control)
Secondary: HRP-conjugated anti-rabbit IgG -
All lanes : Anti-FOXB2 antibody [EPR11476] (ab170897) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : LoVo cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 45 kDa
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab170897 has not yet been referenced specifically in any publications.