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Our Abpromise guarantee covers the use of ab5079 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ChIP||Use at an assay dependent dilution. PubMed: 17000708|
|WB||Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 57 kDa.Can be blocked with Human FOXC1 peptide (ab23069).|
|IHC-P||Use a concentration of 4 - 6 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
Primary incubations were for 1 hour.
Detected by chemiluminescence.
ab5079 at 3ug/ml staining FOXC1 in human kidney tissue section by Immunohistochemistry (Formalin/PFA fixed paraffin-embedded sections). Tissue underwent antigen retrieval in microwave with Tris/EDTA buffer (pH 9.0). The HRP-staining procedure was used for detection.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"