Overview

  • Product name
  • Description
    Rabbit polyclonal to FOXD2
  • Specificity
    This antibody recognizes FOXD2
  • Tested applications
    Suitable for: ELISA, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide: GELPARSGPR APRDVLPHGH EPPAEEAEAD LAEDEEESGG CSDGEPRALA, corresponding to a region within N terminal amino acids 37-86 of Human FOXD2

  • Positive control
    • Jurkat cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab49128 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay was 1:62500.

WB Use a concentration of 1.25 µg/ml. Predicted molecular weight: 49 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • Function
    Probable transcription factor involved in embryogenesis and somatogenesis.
  • Tissue specificity
    Kidney specific.
  • Sequence similarities
    Contains 1 fork-head DNA-binding domain.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Drosphilia Forkhead Homolog Like 17 antibody
    • FKHL 17 antibody
    • Forkhead box protein D2 antibody
    • Forkhead Related Activator 9 antibody
    • Forkhead-related protein FKHL17 antibody
    • Forkhead-related transcription factor 9 antibody
    • FOXD2 antibody
    • FOXD2_HUMAN antibody
    • FREAC 9 antibody
    • FREAC-9 antibody
    see all

Images

  • Lane 1 : Marker
    Lane 2 : Anti-FOXD2 antibody (ab49128) at 1.25 µg/ml

    Lane 1 : As above
    Lane 2 : Jurkat cell lysate at 10 µg with 5% skimmed milk in PBS.

    Secondary
    Lane 2 : HRP conjugated anti-Rabbit IgG at 1/50000 dilution


    Predicted band size : 49 kDa
    Observed band size : 50 kDa (why is the actual band size different from the predicted?)

References

ab49128 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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