Anti-FOXD3 antibody (ab64807)
Key features and details
- Rabbit polyclonal to FOXD3
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-FOXD3 antibody
See all FOXD3 primary antibodies -
Description
Rabbit polyclonal to FOXD3 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse -
Immunogen
A synthesized peptide derived from an internal region of human FOXD3.
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Positive control
- Human heart tissue and extracts from HUVEC and 293 cells
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol, 0.87% Sodium chloride -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab64807 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/1000. Detects a band of approximately 48 kDa (predicted molecular weight: 48 kDa).
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IHC-P |
1/50 - 1/100. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
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Notes |
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WB
1/500 - 1/1000. Detects a band of approximately 48 kDa (predicted molecular weight: 48 kDa). |
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol. |
Target
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Function
Binds to the consensus sequence 5'-A[AT]T[AG]TTTGTTT-3' and acts as a transcriptional repressor. Also acts as a transcriptional activator. Promotes development of neural crest cells from neural tube progenitors. Restricts neural progenitor cells to the neural crest lineage while suppressing interneuron differentiation. Required for maintenance of pluripotent cells in the pre-implantation and peri-implantation stages of embryogenesis. -
Tissue specificity
Expressed in chronic myeloid leukemia, Jurkat T-cell leukemia and teratocarcinoma cell lines, but not in any other cell lines or normal tissues examined. -
Involvement in disease
Defects in FOXD3 are associated with susceptibility to autoimmune disease type 1 (AIS1) [MIM:607836]. AIS1 is a disorder characterized by the association of vitiligo with autoimmune thyroiditis (Hashimoto thyroiditis). -
Sequence similarities
Contains 1 fork-head DNA-binding domain. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 27022 Human
- Entrez Gene: 15221 Mouse
- Omim: 611539 Human
- SwissProt: Q9UJU5 Human
- SwissProt: Q61060 Mouse
- Unigene: 546573 Human
- Unigene: 4758 Mouse
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Alternative names
- AIS1 antibody
- forkhead box D3 antibody
- Forkhead box protein D3 antibody
see all
Images
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All lanes : Anti-FOXD3 antibody (ab64807) at 1/500 dilution
Lane 1 : Extracts of HUVEC cells
Lane 2 : Extracts of 293 cells
Lane 3 : Extracts of 293 cells, plus immunising peptide at 5-10µg
Lysates/proteins at 5 µg per lane.
Predicted band size: 48 kDa
Observed band size: 48 kDa -
Immunohistochemistry analysis of paraffin-embedded human heart tissue using ab64807 at a 1:50 dilution.
Left image untreated.Right image treated with immunising peptide.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (4)
ab64807 has been referenced in 4 publications.
- Liu R et al. FOXD3 and GAB2 as a pair of rivals antagonistically control hepatocellular carcinogenesis. FEBS J 289:4536-4548 (2022). PubMed: 35178865
- Cheng Y et al. FOXD3-induced miR-133a blocks progression and metastasis of colorectal cancer through regulating UBA2. J Cancer 12:6145-6154 (2021). PubMed: 34539887
- Huang J & Chen L IL-1β inhibits osteogenesis of human bone marrow-derived mesenchymal stem cells by activating FoxD3/microRNA-496 to repress wnt signaling. Genesis 55:N/A (2017). PubMed: 28509407
- Douglas GC et al. Trophoblast stem cells: models for investigating trophectoderm differentiation and placental development. Endocr Rev 30:228-40 (2009). PubMed: 19299251