Overview

  • Product nameAnti-FOXD3 antibody - ChIP Grade
    See all FOXD3 primary antibodies
  • Description
    Rabbit polyclonal to FOXD3 - ChIP Grade
  • Tested applicationsSuitable for: WB, ChIPmore details
  • Species reactivity
    Reacts with: Chicken
    Predicted to work with: Mouse, Human
  • Immunogen

    Synthetic peptide derived from N terminal domain of chicken FOXD3 protein

Properties

Applications

Our Abpromise guarantee covers the use of ab67758 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/5000. Predicted molecular weight: 41 kDa.
ChIP Use 1µg for 105 cells.

Target

  • FunctionBinds to the consensus sequence 5'-A[AT]T[AG]TTTGTTT-3' and acts as a transcriptional repressor. Also acts as a transcriptional activator. Promotes development of neural crest cells from neural tube progenitors. Restricts neural progenitor cells to the neural crest lineage while suppressing interneuron differentiation. Required for maintenance of pluripotent cells in the pre-implantation and peri-implantation stages of embryogenesis.
  • Tissue specificityExpressed in chronic myeloid leukemia, Jurkat T-cell leukemia and teratocarcinoma cell lines, but not in any other cell lines or normal tissues examined.
  • Involvement in diseaseDefects in FOXD3 are associated with susceptibility to autoimmune disease type 1 (AIS1) [MIM:607836]. AIS1 is a disorder characterized by the association of vitiligo with autoimmune thyroiditis (Hashimoto thyroiditis).
  • Sequence similaritiesContains 1 fork-head DNA-binding domain.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • AIS1 antibody
    • forkhead box D3 antibody
    • Forkhead box protein D3 antibody
    • foxd3 antibody
    • FOXD3_HUMAN antibody
    • Genesis antibody
    • HFH2 antibody
    • HNF3/FH transcription factor genesis antibody
    • VAMAS2 antibody
    see all

References for Anti-FOXD3 antibody - ChIP Grade (ab67758)

This product has been referenced in:
  • Guo H  et al. The aspirin-induced long non-coding RNA OLA1P2 blocks phosphorylated STAT3 homodimer formation. Genome Biol 17:24 (2016). WB ; Human . Read more (PubMed: 26898989) »

See 1 Publication for this product

Product Wall

Application Western blot
Loading amount 20 µg
Gel Running Conditions Reduced Denaturing (4-15% gradient)
Sample Mouse Tissue lysate - whole (Sciatic nerve)
Specification Sciatic nerve
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Sep 17 2014

Thank you for contacting us.

The human species for antibody ab67758 is predicted which means it has not been tested. However this does not mean it will not work, it might work but we do not have data to confirm it. If you are interested in usi...

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I am very pleased to hear you would still like to accept our offer and test ab67758 in ICC/IF on chicken samples. We have created a new testing program at Abcam called the AbTrial program. There is a slight change as compared with the 100% Abreview Tes...

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I am very pleased to hear you would like to accept our offer and test ab75590 and ab67758 on chicken samples by ICC/IF. These codes will give you: one free primary antibody per code before the expiration date. To redeem this offer, please submit an Abr...

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Thank you for your inquiry.

I contacted the producing labs of these two antibodies. Although the immunogen sequences are confidential, they did an alignment with the sequence you provided.

Unfortunately, both immunogen sequences did...

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Thank you for your reply.
If the free item is the only item on your order, you will not be charged shipping. I hope this helps, please let me know if you have any other questions.

DISCOUNT CODE: ***
Expiration date: August 17th, 2012

I am very pleased to hear you would like to accept our offer and test ab67758 in ChIP. This code will give you: 1 free primary antibody before the expiration date. To redeem this off...

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Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application ChIP
Sample Chicken Cell lysate - whole cell (Trunk half of the embyo)
Specification Trunk half of the embyo
Type Cross-linking (X-ChIP)
Duration of cross-linking step: 10 minute(s) and 0 second(s)
Specification of the cross-linking agent: Formaldehyde
Detection step Real-time PCR
Positive control Chicken embryo trunk samples
Negative control Not available for sample.
Negative control for QPCR: 3'UTR region of my gene of interest.
Username

Mr. Maneeshi Prasad

Verified customer

Submitted Jun 22 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"