Overview

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab5080 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 2 - 4 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Target

  • FunctionProbable transcription factor. Could be involved in thyroid gland organogenesis.
  • Tissue specificityDetected in adult brain, placenta, lung, liver, skeletal muscle, kidney, pancreas, heart, colon, small intestine testis and thymus. Expression was strongest in heart and pancreas.
  • Involvement in diseaseDefects in FOXE1 are the cause of Bamforth-Lazarus syndrome (BLS) [MIM:241850]. BLS is associated with thyroid agenesis, cleft palate and choanal atresia.
  • Sequence similaritiesContains 1 fork-head DNA-binding domain.
  • Post-translational
    modifications
    Phosphorylated.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • FKH L15 antibody
    • FKHL 15 antibody
    • FKHL15 antibody
    • Forkhead (Drosophila) like 15 antibody
    • Forkhead box E1 antibody
    • Forkhead box E2 antibody
    • Forkhead box protein E1 antibody
    • Forkhead box protein E2 antibody
    • Forkhead drosophila homolog like 15 antibody
    • Forkhead like 15 antibody
    • Forkhead related protein FKHL 15 antibody
    • Forkhead related protein FKHL15 antibody
    • Forkhead-related protein FKHL15 antibody
    • FOX E1 antibody
    • FOX E2 antibody
    • FOXE 1 antibody
    • FOXE 2 antibody
    • FOXE1 antibody
    • FOXE1_HUMAN antibody
    • FOXE2 antibody
    • HFKH 4 antibody
    • HFKH4 antibody
    • HFKL 5 antibody
    • HFKL5 antibody
    • HNF-3/fork head-like protein 5 antibody
    • Thyroid transcription factor 2 antibody
    • TITF 2 antibody
    • TITF2 antibody
    • TTF 2 antibody
    • TTF-2 antibody
    • TTF2 antibody
    see all

Anti-FOXE1 antibody images

  • ab5080 (2µg/ml) staining of paraffin embedded Human Thyroid Gland staining in thyrocytes. Steamed antigen retrieval with Tris/EDTA buffer pH 9, HRP-staining

  • ab5080 (2.5 µg/ml) staining FOXE1 in paraffin-embedded sections of human testis. Steamed antigen retrieval with citrate buffer (pH 6) was performed.

References for Anti-FOXE1 antibody (ab5080)

This product has been referenced in:
  • Bullock M  et al. Association of FOXE1 polyalanine repeat region with papillary thyroid cancer. J Clin Endocrinol Metab 97:E1814-9 (2012). EMSA . Read more (PubMed: 22736773) »
  • Zhang P  et al. Immunohistochemical analysis of thyroid-specific transcription factors in thyroid tumors. Pathol Int 56:240-5 (2006). IHC-P ; Human . Read more (PubMed: 16669872) »

See all 2 Publications for this product

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (thyroid)
Specification thyroid
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: citrate
Permeabilization Yes - 0.1% triton
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: TA°C
Username

Dr. Aurore Simon-Carré

Verified customer

Submitted May 01 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"