Recombinant
RabMAb

Anti-FOXF1 antibody [EPR7971] (ab168383)

Overview

  • Product name
    Anti-FOXF1 antibody [EPR7971]
    See all FOXF1 primary antibodies
  • Description
    Rabbit monoclonal [EPR7971] to FOXF1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, ICC/IF, Flow Cytmore details
    Unsuitable for: IHC-P or IP
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human FOXF1. The exact sequence is proprietary.
    Database link: Q12946

  • Positive control
    • Human fetal lung and HeLa lysates; HeLa cells.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab168383 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Predicted molecular weight: 40 kDa.
ICC/IF 1/250 - 1/500.
Flow Cyt 1/100 - 1/500.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

  • Application notes
    Is unsuitable for IHC-P or IP.
  • Target

    • Function
      Probable transcription activator for a number of lung-specific genes.
    • Tissue specificity
      Lung and placenta.
    • Involvement in disease
      Defects in FOXF1 are the cause of alveolar capillary dysplasia with misalignment of pulmonary veins (ACDMPV) [MIM:265380]. ACDMPV is a rare malformation due to abnormal development of the capillary vascular system in the lungs. Histologically, it is characterized by failure of formation and ingrowth of alveolar capillaries, medial muscular thickening of small pulmonary arterioles with muscularization of the intraacinar arterioles, thickened alveolar walls, and anomalously situated pulmonary veins running alongside pulmonary arterioles and sharing the same adventitial sheath. Less common features include a reduced number of alveoli and a patchy distribution of the histopathologic changes. Affected infants present with respiratory distress and the disease is fatal within the newborn period. Additional features include multiple congenital anomalies affecting the cardiovascular, gastrointestinal, genitourinary, and musculoskeletal systems, as well as disruption of the normal right-left asymmetry of intrathoracic or intraabdominal organs. ACDMPV is a rare cause of persistent pulmonary hypertension of the newborn, an abnormal physiologic state caused by failure of transition of the pulmonary circulation from the high pulmonary vascular resistance of the fetus to the low pulmonary vascular resistance of the newborn.
    • Sequence similarities
      Contains 1 fork-head DNA-binding domain.
    • Domain
      Activation domains C-terminal of (and distinct from) the forkhead domains are necessary for transcriptional activation.
    • Cellular localization
      Nucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • AI450827 antibody
      • FKH L5 antibody
      • FKHL 5 antibody
      • FKHL5 antibody
      • Forkhead (Drosophila) like 5 antibody
      • Forkhead box F1 antibody
      • Forkhead box protein F1 antibody
      • Forkhead drosophila homolog like 5 antibody
      • Forkhead like 5 antibody
      • Forkhead related activator 1 antibody
      • Forkhead related protein FKHL5 antibody
      • Forkhead related transcription factor 1 antibody
      • Forkhead-related activator 1 antibody
      • Forkhead-related protein FKHL5 antibody
      • Forkhead-related transcription factor 1 antibody
      • FOX F1 antibody
      • FOXF 1 antibody
      • foxf1 antibody
      • FOXF1_HUMAN antibody
      • FREAC 1 antibody
      • FREAC-1 antibody
      • FREAC1 antibody
      • HFH 8 antibody
      • MGC105125 antibody
      see all

    Images

    • All lanes : Anti-FOXF1 antibody [EPR7971] (ab168383) at 1/1000 dilution

      Lane 1 : Human fetal lung lysate
      Lane 2 : HeLa lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size: 40 kDa

    • Immunofluorescent analysis of HeLa cells labeling FOXF1 with ab168383 at 1/250 dilution.
    • Flow cytometric analysis of permeabilized HeLa cells labeling FOXF1 (red) with ab168383 at 1/100 dilution, or a rabbit IgG (negative) (green).

    References

    ab168383 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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