Anti-FOXG1 antibody - ChIP Grade (ab18259)
- Datasheet
- Specific References (19)
- Protocols
Overview
- Product nameAnti-FOXG1 antibody - ChIP Grade
See all FOXG1 primary antibodies - DescriptionRabbit polyclonal to FOXG1 - ChIP Grade
- Tested applicationsSuitable for: WB, ChIP, IHC-P, IHC-Fr, ICC/IFmore details
- Species reactivityReacts with: Mouse, Rat, Human, Xenopus laevis
- Immunogen
Synthetic peptide corresponding to Human FOXG1 aa 400 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin.
Database link: P55316
(Peptide available asab19644) - Positive control
- This antibody gave a positive signal in Human, Mouse and Rat brain tissue lysates. IHC-P: FFPE mouse brain E14.
Properties
- FormLiquid
- Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
- Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4 -
Concentration information loading... - PurityImmunogen affinity purified
- ClonalityPolyclonal
- IsotypeIgG
- Research areas
Associated products
- Compatible Secondaries
- Immunizing Peptide (Blocking)
Applications
Our Abpromise guarantee covers the use of ab18259 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use a concentration of 1 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 50 kDa). | |
| ChIP | Use at an assay dependent concentration. | |
| IHC-P | Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. | |
| IHC-Fr | 1/1000. | |
| ICC/IF | Use at an assay dependent concentration. |
Target
- FunctionTranscription repression factor which plays an important role in the establishment of the regional subdivision of the developing brain and in the development of the telencephalon.
- Tissue specificityExpression is restricted to the neurons of the developing telencephalon.
- Involvement in diseaseDefects in FOXG1 are the cause of congenital variant of Rett syndrome (RTTCV) [MIM:613454]. RTTCV is a severe neurodevelopmental disorder with features of classic Rett syndrome but earlier onset in the first months of life. Clinical features include progressive microcephaly, hypotonia, irresponsiveness and irritability in the neonatal period, mental retardation, psychomotor regression and stereotypical movements.
- Sequence similaritiesContains 1 fork-head DNA-binding domain.
- Cellular localizationNucleus.
- Information by UniProt
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Database links
- Entrez Gene: 2290 Human
- Entrez Gene: 15228 Mouse
- Entrez Gene: 24370 Rat
- Entrez Gene: 373732 Xenopus laevis
- Omim: 164874 Human
- SwissProt: P55316 Human
- SwissProt: Q60987 Mouse
- SwissProt: Q00939 Rat
see all -
Alternative names
- BF-1 antibody
- BF-2 antibody
- BF1 antibody
see all
Anti-FOXG1 antibody - ChIP Grade images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXG1 antibody - ChIP Grade (ab18259)IHC image of FoxG1 staining in mouse brain E14 formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol B. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab18259, 0.5 µg/ml, for 15 mins at room temperature. A goat anti-rabbit biotinylated secondary antibody was used to detect the primary, and visualized using an HRP conjugated ABC system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Staining revealed in telencephalon (but not diencephalon) as expected.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
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Immunocytochemistry/ Immunofluorescence - Anti-FOXG1 antibody - ChIP Grade (ab18259)This image is courtesy of an anonymous Abreviewab18259 staining FOXG1 in Rat embryonic cortex cells overexpressing FOXG1 by ICC/IF (Immunocytochemistry/ immunofluorescence). Cells were fixed with formaldehyde and blocked with 1o% serum for 1 hour at 25°C. Samples were incubated with primary antibody (1/500) for 24 hours at 4°C. A Cy3®-conjugated anti-rabbit IgG polyclonal was used as the secondary antibody (1/500).
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Western blot - Anti-FOXG1 antibody - ChIP Grade (ab18259)All lanes : Anti-FOXG1 antibody - ChIP Grade (ab18259) at 1 µg/ml
Lane 1 : Human brain tissue lysate - total protein (ab29466)
Lane 2 : Brain (Mouse) Tissue Lysate
Lane 3 : E10 Mouse Embryo Brain Tissue Lysate
Lane 4 : Brain (Rat) Tissue Lysate
Lane 5 : Human spleen tissue lysate - total protein (negative control)
Lysates/proteins at 10 µg per lane.
Secondary
Goat Anti-Rabbit IgG H&L (HRP) at 1/50000 dilution
Developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 50 kDa
Observed band size : 50 kDa
Additional bands at : 155 kDa,52 kDa. We are unsure as to the identity of these extra bands.
Exposure time : 3 minutesThis blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% Milk before being incubated with ab18259 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution ab133406.
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Western blot - Anti-FOXG1 antibody - ChIP Grade (ab18259)All lanes : Anti-FOXG1 antibody - ChIP Grade (ab18259) at 1 µg/ml
Lane 1 : Human brain tissue lysate - total protein (ab29466)
Lane 2 : Brain (Mouse) Tissue Lysate
Lane 3 : Brain (Rat) Tissue Lysate
Lane 4 : Human brain tissue lysate - total protein (ab29466) withHuman FOXG1 peptide (ab19644) at 1 µg/ml
Lane 5 : Brain (Mouse) Tissue Lysate withHuman FOXG1 peptide (ab19644) at 1 µg/ml
Lane 6 : Brain (Rat) Tissue Lysate withHuman FOXG1 peptide (ab19644) at 1 µg/ml
Lysates/proteins at 10 µg per lane.
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 50 kDa
Observed band size : 50 kDa
Exposure time : 1 minuteab18259 detects a band of ~50kDa in brain lysates. This band is blocked by addition of the immunizing peptide ab19644 which suggests that is specific for FOXG1.
This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab18259 overnight at 4°C. Antibody binding was detected using an anti-rabbit HRP secondary antibody, and visualised using ECL development solution.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXG1 antibody - ChIP Grade (ab18259)This image is courtesy of Vassiliki Fotaki, University of Edinburghab18259 staining Foxg1 (1/50) in the telencephalon (but not the diencephalon) as expected. DAB-immunohistochemistry was performed on embryonic (E13.5) mouse brain (coronal paraffin-embedded sections) after microwave treatment with 10mM sodium citrate.
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Immunocytochemistry/ Immunofluorescence - Anti-FOXG1 antibody - ChIP Grade (ab18259)ab18259 staining FOXG1 in human A549 cells by immunocytochemistry/immunofluorescence.Cells were fixed in paraformaldehyde, permeabilized using 0.25% Triton, blocked with 1% BSA for 1 hour at room temperature and then incubated with ab18259 at a 1/400 dilution for 1 hour. The secondary used was an Alexa-Fluor 488 conjugated goat polyclonal used at a 1/250 dilution.
References for Anti-FOXG1 antibody - ChIP Grade (ab18259)
This product has been referenced in:
- Subramanian L et al. Dynamic behaviour of human neuroepithelial cells in the developing forebrain. Nat Commun 8:14167 (2017). IF ; Human . Read more (PubMed: 28139695) »
- Xu CC et al. Abnormal mitochondrial transport and morphology as early pathological changes in human models of spinal muscular atrophy. Dis Model Mech 9:39-49 (2016). Human . Read more (PubMed: 26586529) »
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"