Anti-FOXI1 antibody (ab20454)
Key features and details
- Goat polyclonal to FOXI1
- Suitable for: WB, IHC-P, ChIP, IHC-Fr
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-FOXI1 antibody
See all FOXI1 primary antibodies -
Description
Goat polyclonal to FOXI1 -
Host species
Goat -
Specificity
This antibody is expected to recognise both reported human isoforms.
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Tested applications
Suitable for: WB, IHC-P, ChIP, IHC-Frmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human FOXI1 aa 350 to the C-terminus. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: Q12951 -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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ChIP Related Products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab20454 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use at an assay dependent concentration. Predicted molecular weight: 41 kDa.
Primary incubation 1 hour at room temperature. |
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IHC-P | (1) |
Use at an assay dependent concentration. PubMed: 21303954
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ChIP |
Use at an assay dependent concentration. PubMed: 20972246
|
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IHC-Fr |
Use at an assay dependent concentration. PubMed: 16932748
An antigen retrieval method using SDS is described in PubMed 16932748 and PubMed 9072183. |
Notes |
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WB
Use at an assay dependent concentration. Predicted molecular weight: 41 kDa. Primary incubation 1 hour at room temperature. |
IHC-P
Use at an assay dependent concentration. PubMed: 21303954 |
ChIP
Use at an assay dependent concentration. PubMed: 20972246 |
IHC-Fr
Use at an assay dependent concentration. PubMed: 16932748 An antigen retrieval method using SDS is described in PubMed 16932748 and PubMed 9072183. |
Target
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Function
Transcriptional activator required for the development of normal hearing, sense of balance and kidney function. Required for the expression of SLC26A4/PDS, JAG1 and COCH in a subset of epithelial cells and the development of the endolymphatic system in the inner ear. Also required for the expression of SLC4A1/AE1, SLC4A9/AE4, ATP6V1B1 and the differentiation of intercalated cells in the epithelium of distal renal tubules. -
Tissue specificity
Expressed in kidney. -
Sequence similarities
Contains 1 fork-head DNA-binding domain. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 2299 Human
- Omim: 601093 Human
- SwissProt: Q12951 Human
- Unigene: 87236 Human
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Alternative names
- FKH10 antibody
- FKHL10 antibody
- Forkhead (Drosophila) like 10 antibody
see all
Images
Protocols
Datasheets and documents
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Datasheet download
References (11)
ab20454 has been referenced in 11 publications.
- Xue X et al. Tumour cells are sensitised to ferroptosis via RB1CC1-mediated transcriptional reprogramming. Clin Transl Med 12:e747 (2022). PubMed: 35220675
- Montoro DT et al. A revised airway epithelial hierarchy includes CFTR-expressing ionocytes. Nature 560:319-324 (2018). PubMed: 30069044
- Plasschaert LW et al. A single-cell atlas of the airway epithelium reveals the CFTR-rich pulmonary ionocyte. Nature 560:377-381 (2018). PubMed: 30069046
- Honda K et al. Molecular architecture underlying fluid absorption by the developing inner ear. Elife 6:N/A (2017). IHC ; Mouse . PubMed: 28994389
- Björkgren I et al. Targeted inactivation of the mouse epididymal beta-defensin 41 alters sperm flagellar beat pattern and zona pellucida binding. Mol Cell Endocrinol 427:143-54 (2016). PubMed: 26987518