Overview

  • Product nameAnti-FOXI1 antibody - ChIP Grade
    See all FOXI1 primary antibodies
  • Description
    Goat polyclonal to FOXI1 - ChIP Grade
  • SpecificityThis antibody is expected to recognise both reported human isoforms
  • Tested applicationsSuitable for: WB, IHC-Fr, IHC-P, ChIPmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide corresponding to Human FOXI1 aa 366-378 (C terminal).
    Sequence:

    TSGVLYPREGTEV


    Database link: Q12951

Properties

Applications

Our Abpromise guarantee covers the use of ab20454 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent dilution. PubMed: 20972246
IHC-Fr 1/1000. PubMed: 16932748
IHC-P Use at an assay dependent dilution. PubMed: 21303954
ChIP Use at an assay dependent dilution. PubMed: 20972246

Target

  • FunctionTranscriptional activator required for the development of normal hearing, sense of balance and kidney function. Required for the expression of SLC26A4/PDS, JAG1 and COCH in a subset of epithelial cells and the development of the endolymphatic system in the inner ear. Also required for the expression of SLC4A1/AE1, SLC4A9/AE4, ATP6V1B1 and the differentiation of intercalated cells in the epithelium of distal renal tubules.
  • Tissue specificityExpressed in kidney.
  • Sequence similaritiesContains 1 fork-head DNA-binding domain.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • FKH10 antibody
    • FKHL10 antibody
    • Forkhead (Drosophila) like 10 antibody
    • Forkhead box I1 antibody
    • Forkhead box protein I1 antibody
    • Forkhead like 10 antibody
    • Forkhead related activator 6 antibody
    • Forkhead related transcription factor 6 antibody
    • Forkhead-related protein FKHL10 antibody
    • Forkhead-related transcription factor 6 antibody
    • FOXI1 antibody
    • FOXI1_HUMAN antibody
    • FREAC 6 antibody
    • FREAC-6 antibody
    • FREAC6 antibody
    • Hepatocyte nuclear factor 3 forkhead homolog 3 antibody
    • HFH 3 antibody
    • HFH-3 antibody
    • HFH3 antibody
    • HNF 3/fork head homolog 3 antibody
    • HNF-3 fork-head homolog 3 antibody
    • HNF-3/fork-head homolog 3 antibody
    • Human HNF-3 fork-head homolog-3 HFH-3 mRNA complete cds antibody
    • MGC34197 antibody
    see all

Anti-FOXI1 antibody - ChIP Grade images

  • ab20454 at 2-4µg/ml, staining FOXI1 in Human Testis tissue sections by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Image shows primarily nuclear staining in the primary spermatocytes. Steamed antigen retrieval with pH 6 citrate buffer was performed, and HRP-staining was used.

     

     

References for Anti-FOXI1 antibody - ChIP Grade (ab20454)

This product has been referenced in:
  • Guo Q  et al. Adam10 Mediates the Choice between Principal Cells and Intercalated Cells in the Kidney. J Am Soc Nephrol N/A:N/A (2014). Read more (PubMed: 24904084) »
  • Trepiccione F  et al. Evaluation of cellular plasticity in the collecting duct during the recovery of lithium-induced nephrogenic diabetes insipidus. Am J Physiol Renal Physiol N/A:N/A (2013). IHC . Read more (PubMed: 23825070) »

See all 5 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (epididymis)
Specification epididymis
Fixative Bouin's solution
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: itrate buffer
Permeabilization No
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 1.5% · Temperature: 25°C
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Submitted Apr 30 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"