Overview

  • Product nameAnti-FOXJ2 antibody
    See all FOXJ2 primary antibodies
  • Description
    Rabbit polyclonal to FOXJ2
  • Specificityab22857 reacts with Forkhead box protein J2 (FOXJ2).
  • Tested applicationsSuitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Cow, Dog
  • Immunogen

    A region within synthetic peptide: AGASGRESAE GPPPLYNTNH DFKFSYSEIN FQDLSWSFRN LYKSMLEKSS, corresponding to internal sequence amino acids 218-267 of Human FOXJ2

  • Positive control
    • HepG2 cell lysate and human stomach

Properties

Applications

Our Abpromise guarantee covers the use of ab22857 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 2 µg/ml. Detects a band of approximately 62 kDa (predicted molecular weight: 62 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use at an assay dependent concentration.

Target

  • FunctionTranscriptional activator. Able to bind to two different type of DNA binding sites. Isoform FOXJ2.L behaves as a more potent transactivator than FOXJ2.S.
  • Tissue specificityWidely expressed.
  • Sequence similaritiesContains 1 fork-head DNA-binding domain.
  • Post-translational
    modifications
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • FHX antibody
    • Fork head homologous X antibody
    • Forkhead box J2 antibody
    • Forkhead box protein J2 antibody
    • FOXJ2 antibody
    • FOXJ2_HUMAN antibody
    see all

Anti-FOXJ2 antibody images

  • Anti-FOXJ2 antibody (ab22857) at 2 µg/ml + HepG2 cell lysate

    Secondary
    HRP conjugated anti-Rabbit IgG should be diluted in 1/50,000 - 100,000.

    Predicted band size : 62 kDa
    Observed band size : 62 kDa
  • ab22857, at 4 µg/ml, staining human FOXJ2 in human Stomach by Immunohistochemistry, Paraffin embedded tissue. Positive stained cells, indicated with arrows, are epithelial cells of fundic gland. Magnification 400 x.

References for Anti-FOXJ2 antibody (ab22857)

ab22857 has not yet been referenced specifically in any publications.

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