Overview

  • Product name
  • Description
    Rabbit polyclonal to FOXK1
  • Tested applications
    Suitable for: WB, IP, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Horse, Guinea pig, Cow, Pig, Chimpanzee, Rhesus monkey, Gorilla
  • Immunogen

    Synthetic peptide corresponding to a region between residues 683 and 733 of human FOXK1 using the numbering given in entry NP_001032242.1.

  • Positive control
    • Whole cell lysate from HeLa cells

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.09% Sodium Azide
    Constituents: 0.1% BSA, Tris buffered saline
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    ab85999 was affinity purified using an epitope specific to FOXK1 immobilized on solid support.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab85999 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 75 kDa.
IP Use at 2-5 µg/mg of lysate.
IHC-P 1/200 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function
    Transcriptional regulator that binds to the upstream enhancer region (CCAC box) of myoglobin gene. Has a role in myogenic differentiation and in remodeling processes of adult muscles that occur in response to physiological stimuli.
  • Tissue specificity
    Expressed both developing and adult tissues. In adults, significant expression is seen in tumors of the brain, colon and lymph node.
  • Sequence similarities
    Contains 1 FHA domain.
    Contains 1 fork-head DNA-binding domain.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • A630048H08Rik antibody
    • AI463295 antibody
    • ENSMUSG00000075577 antibody
    • FHX antibody
    • Fork head antibody
    • Forkhead box protein K1 antibody
    • FOXJ2 forkhead factor antibody
    • FOXK1 antibody
    • FOXK1_HUMAN antibody
    • Gm10868 antibody
    • MNF antibody
    • Myocyte nuclear factor antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma (left) and mouse teratoma (right) tissues labelling FOXK1 with ab85999 at 1/1000 (0.2µg/ml) and 1/200 (1µg/ml). Detection: DAB.
  • All lanes : Anti-FOXK1 antibody (ab85999) at 0.04 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : 293T whole cell lysate at 50 µg

    Developed using the ECL technique

    Predicted band size : 75 kDa


    Exposure time : 30 seconds
  • Detection of FOXK1 by Western blot of Immunprecipitate.
    ab85999, at 1 µg/ml, staining FOXK1 in HeLa whole cell lysate immunoprecipitated using ab85999 at 3 µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
    Detection: Chemiluminescence with an exposure time of 3 seconds.

References

ab85999 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab85999.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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