Overview

  • Product name
  • Description
    Rabbit polyclonal to FOXL1
  • Tested applications
    Suitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    KLH conjugated synthetic peptide selected from the central region of Human FOXL1.

  • Positive control
    • CHO cell lysate or mouse kidney tissue lysate. This antibody gave a positive result when used in the following methanol fixed cell line: HeLa.

Properties

Applications

Our Abpromise guarantee covers the use of ab95286 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100 - 1/500. Predicted molecular weight: 36 kDa.
ICC/IF 1/200.

Target

  • Sequence similarities
    Contains 1 fork-head DNA-binding domain.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • FKH 6 antibody
    • FKH6 antibody
    • FKHL 11 antibody
    • FKHL11 antibody
    • Forkhead (Drosophila) like 11 antibody
    • Forkhead box L1 antibody
    • Forkhead box protein L1 antibody
    • Forkhead related protein FKHL11 antibody
    • Forkhead related transcription factor 7 antibody
    • Forkhead-related protein FKHL11 antibody
    • Forkhead-related transcription factor 7 antibody
    • FOX L1 antibody
    • FOXL 1 antibody
    • Foxl1 antibody
    • FOXL1_HUMAN antibody
    • FREAC 7 antibody
    • FREAC-7 antibody
    • FREAC7 antibody
    see all

Images

  • ICC/IF image of ab95286 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1% BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab95286 at 1/200 dilution overnight at +4°C. The secondary antibody (green) was DyLight® 488 Goat anti-Rabbit IgG (H+L) (ab96899) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Anti-FOXL1 antibody (ab95286) at 1/100 dilution + CHO cell line lysates at 35 µg

    Predicted band size : 36 kDa
  • Anti-FOXL1 antibody (ab95286) at 1/100 dilution + mouse kidney tissue lysates at 35 µg

    Predicted band size : 36 kDa

References

ab95286 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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