Anti-FOXL2 antibody [262C1a] (ab58622)

Overview

  • Product nameAnti-FOXL2 antibody [262C1a]
    See all FOXL2 primary antibodies
  • Description
    Mouse monoclonal [262C1a] to FOXL2
  • Tested applicationsSuitable for: WB, Dot blotmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment: NSIRHNLSLN ECFIKVPREG GGERKGNYWT LDPACEDMFE KGNYRRRRRM KRPFRPPPAH FQPGKGLFGA GGAAGGCGVA GAGADGYGYL APPKYLQSGF LN, corresponding to amino acids 100-201 of Human FOXL2

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage bufferPreservative: 0.05% Sodium Azide
    Constituents: 1% BSA, PBS (8.0mM Sodium phosphate, 3.0mM Potassium chloride, 140mM Sodium chloride, 1.5mM Potassium phosphate), pH 7.4
  • Concentration information loading...
  • PurityProtein G purified
  • Purification notesPurified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum and filtered through a 0.22µm membrane.
  • ClonalityMonoclonal
  • Clone number262C1a
  • IsotypeIgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab58622 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent dilution. Predicted molecular weight: 42 kDa.
Dot blot Use at an assay dependent dilution.

Target

  • FunctionTranscriptional regulator. Critical factor essential for ovary differentiation and maintenance, and repression of the genetic program for somatic testis determination. Prevents trans-differentiation of ovary to testis throught transcriptional repression of the Sertoli cell-promoting gene SOX9 (By similarity). Has apoptotic activity in ovarian cells. Suppresses ESR1-mediated transcription of PTGS2/COX2 stimulated by tamoxifen (By similarity). Is a regulator of CYP19 expression (By similarity). Participates in SMAD3-dependent transcription of FST via the intronic SMAD-binding element (By similarity). Is a transcriptional repressor of STAR. Activates SIRT1 transcription under cellular stress conditions. Activates transcription of OSR2.
  • Tissue specificityIn addition to its expression in the developing eyelid, it is transcribed very early in somatic cells of the developing gonad (before sex determination) and its expression persists in the follicular cells of the adult ovary.
  • Involvement in diseaseDefects in FOXL2 are a cause of blepharophimosis, ptosis, and epicanthus inversus syndrome (BPES) [MIM:110100]; also known as blepharophimosis syndrome. It is an autosomal dominant disorder characterized by eyelid dysplasia, small palpebral fissures, drooping eyelids and a skin fold running inward and upward from the lower lid. In type I BPSE (BPES1) eyelid abnormalities are associated with female infertility. Affected females show an ovarian deficit due to primary amenorrhea or to premature ovarian failure (POF). In type II BPSE (BPES2) affected individuals show only the eyelid defects. There is a mutational hotspot in the region coding for the poly-Ala domain, since 30% of all mutations in the ORF lead to poly-Ala expansions, resulting mainly in BPES type II.
    Defects in FOXL2 are a cause of premature ovarian failure type 3 (POF3) [MIM:608996]. An ovarian disorder defined as the cessation of ovarian function under the age of 40 years. It is characterized by oligomenorrhea or amenorrhea, in the presence of elevated levels of serum gonadotropins and low estradiol.
  • Sequence similaritiesContains 1 fork-head DNA-binding domain.
  • Post-translational
    modifications
    Sumoylated by SUMO1; sumoylation is required for transcriptional repression activity.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Blepharophimosis antibody
    • Blepharophimosis epicanthus inversus and ptosis 1 antibody
    • Blepharophimosis epicanthus inversus and ptosis antibody
    • BPES 1 antibody
    • BPES antibody
    • BPES1 antibody
    • Epicanthus inversus and ptosis 1 antibody
    • Forkhead box L2 antibody
    • Forkhead box protein L2 antibody
    • Forkhead transcription factor FOXL2 antibody
    • FOX L2 antibody
    • FOXL 2 antibody
    • FOXL2 antibody
    • FOXL2_HUMAN antibody
    • PFRK antibody
    • PINTO antibody
    • PITUITARY FORKHEAD FACTOR antibody
    • POF 3 antibody
    • POF3 antibody
    see all

Anti-FOXL2 antibody [262C1a] images

  • Anti-FOXL2 antibody [262C1a] (ab58622) + immunogen (recombinant fragment)

    Predicted band size : 42 kDa

References for Anti-FOXL2 antibody [262C1a] (ab58622)

ab58622 has not yet been referenced specifically in any publications.

Product Wall

Thank you for your reply.
We do not have immunohistochemical staining facilities available for testing customer-submitted samples.
You could purchase the antibody and test it in IHC as part of our AbTrial program where you purchase the antibo...

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Thank you for contacting us and for your interest in our products.
As far as we are aware, ab58622 has never been tested in IHC. All tested applications are specified on our datasheets, and these are updated as soon as any new information is broug...

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Abcam has not validated the combination of species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Turtle Cell (Gonad)
Specification Gonad
Fixative paraformaldehyde and methanol
Permeabilization Yes - 0.2% triton-x-100
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 22°C
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Submitted Oct 29 2009

Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Mouse Tissue lysate - whole (adult ovary)
Loading amount 50 µg
Specification adult ovary
Gel Running Conditions Reduced Denaturing (4-15%)
Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
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Submitted Oct 29 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"