Our Abpromise guarantee covers the use of ab5096 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use at an assay dependent concentration.|
|IHC-P||Use a concentration of 2 - 4 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||Use a concentration of 0.5 - 1.5 µg/ml. Detects a band of approximately 45-50 kDa (predicted molecular weight: 43 kDa).|
Immunohistochemical analysis of paraffin embedded Human Ovary tissue labeling FOXL2 with ab5096 at 2 ug/ml. Tissue underwent antigen retrieval in steam with citrate buffer (pH 6.0). The HRP-staining procedure was used for detection.